PDF(Pubmed)
Abstract：
UNASSIGNED: Oats, a highly nutritious cereal known for their health benefits, contain various macromolecules of significant biological value, including abundant and highly digestible proteins. Despite their importance, oat proteins have not been extensively studied. Here, we present a complete set of the expressed globulins genes, which code for the main storage protein in oats as well as their chromosomal positions.
UNASSIGNED: Published expressed sequence tags for globulins were used as queries in the Sang oat genome. In addition, globulin proteins were fractionated from oat flour by solvent extraction based on differential solubility with other classes of cereal proteins. The protein fractions were separated by gel electrophoresis and analyzed by tandem mass spectrometry to confirm their identity and expression in seed.
UNASSIGNED: In total 32 globulin gene sequences were identified on the oat genome. Out of these, the expression on RNA level could be confirmed and 27 were also detected as expressed proteins by MS. Our results provide the most extensive set of salt-soluble oat globulin sequences to date, paving the way for further understanding their implications for human nutrition. In addition, a simple methodology to fractionate oat proteins is presented.
UNASSIGNED: Published expressed sequence tags for globulins were used as queries in the Sang oat genome. In addition, globulin proteins were fractionated from oat flour by solvent extraction based on differential solubility with other classes of cereal proteins. The protein fractions were separated by gel electrophoresis and analyzed by tandem mass spectrometry to confirm their identity and expression in seed.
UNASSIGNED: In total 32 globulin gene sequences were identified on the oat genome. Out of these, the expression on RNA level could be confirmed and 27 were also detected as expressed proteins by MS. Our results provide the most extensive set of salt-soluble oat globulin sequences to date, paving the way for further understanding their implications for human nutrition. In addition, a simple methodology to fractionate oat proteins is presented.
摘要：
■燕麦，一种以健康益处而闻名的高营养谷物，含有各种具有重要生物学价值的大分子，包括丰富和高度易消化的蛋白质。尽管它们很重要,燕麦蛋白尚未被广泛研究。这里,我们提出了一套完整的表达球蛋白基因，编码燕麦中的主要贮藏蛋白及其染色体位置。
■公布的球蛋白表达序列标签用作Sang燕麦基因组中的查询。此外,根据与其他谷物蛋白的溶解度差异，通过溶剂萃取从燕麦面粉中分离出球蛋白。通过凝胶电泳分离蛋白质级分,并通过串联质谱分析以确认它们在种子中的身份和表达。
■在燕麦基因组上总共鉴定了32个球蛋白基因序列。在这些中，可以证实RNA水平的表达，并且MS还检测到27种表达蛋白。我们的结果提供了迄今为止最广泛的盐溶性燕麦球蛋白序列，为进一步了解它们对人类营养的影响铺平了道路。此外,提出了一种简单的方法来分级分离燕麦蛋白。
■公布的球蛋白表达序列标签用作Sang燕麦基因组中的查询。此外,根据与其他谷物蛋白的溶解度差异，通过溶剂萃取从燕麦面粉中分离出球蛋白。通过凝胶电泳分离蛋白质级分,并通过串联质谱分析以确认它们在种子中的身份和表达。
■在燕麦基因组上总共鉴定了32个球蛋白基因序列。在这些中，可以证实RNA水平的表达，并且MS还检测到27种表达蛋白。我们的结果提供了迄今为止最广泛的盐溶性燕麦球蛋白序列，为进一步了解它们对人类营养的影响铺平了道路。此外,提出了一种简单的方法来分级分离燕麦蛋白。
