PDF(Pubmed)
Abstract：
UNASSIGNED: Cisplatin, an anti-cancer chemotherapy drug, has nephrotoxic effects. Thymus caramanicus Jalas (TCJ) has antioxidant effects due to its main components.
UNASSIGNED: In the current research, we assessed the impact of TCJ extract and its main compound on cisplatin-induced nephrotoxicity in mice.
UNASSIGNED: Forty-two male mice were used in the study. Depending on their group, the animals received saline, carvacrol (10 mg/kg), or TCJ extract (50, 100, and 150 mg/kg) for 10 days. On the fifth day, mice received cisplatin (7.5 mg/kg, i.p.). After 10 days, serum creatinine (Cr) and blood urea nitrogen (BUN) levels were measured. Additionally, malondialdehyde (MDA) and glutathione (GSH) contents, as well as the activity levels of superoxide dismutase (SOD), catalase, and glutathione peroxidase (GPx), and total antioxidant capacity (TAC) were measured in the kidney tissues. The western blotting method was used to determine the kidney\'s expression of cleaved caspase-3, Bax, Bcl-2, nuclear factor kappa-B (NF-κB), and tumor necrosis factor-alpha (TNF-α). Kidney tissue damage score (KTDS) was assessed using the hematoxylin-eosin (H&E) staining method.
UNASSIGNED: Cisplatin significantly increased serum Cr, KTDS, MDA, BUN levels, NF-κB, TNF-α, cleaved caspase-3, and Bax protein expression in the cisplatin group compared to the control group (P < 0.01). Additionally, cisplatin significantly decreased the kidney tissue\'s TAC and GSH content, activity levels of SOD, catalase, and GPx indicators, and expression of Bcl-2 protein (P < 0.05). TCJ and carvacrol significantly ameliorated these indicators in the cisplatin + TCJ (150 mg/kg) and cisplatin + carvacrol (10 mg/kg) groups compared to the cisplatin group (P < 0.05).
UNASSIGNED: TCJ (150 mg/kg) and its main component, carvacrol, could somewhat reduce cisplatin-induced nephrotoxicity through their anti-inflammatory, antioxidant, and anti-apoptotic effects.
UNASSIGNED: In the current research, we assessed the impact of TCJ extract and its main compound on cisplatin-induced nephrotoxicity in mice.
UNASSIGNED: Forty-two male mice were used in the study. Depending on their group, the animals received saline, carvacrol (10 mg/kg), or TCJ extract (50, 100, and 150 mg/kg) for 10 days. On the fifth day, mice received cisplatin (7.5 mg/kg, i.p.). After 10 days, serum creatinine (Cr) and blood urea nitrogen (BUN) levels were measured. Additionally, malondialdehyde (MDA) and glutathione (GSH) contents, as well as the activity levels of superoxide dismutase (SOD), catalase, and glutathione peroxidase (GPx), and total antioxidant capacity (TAC) were measured in the kidney tissues. The western blotting method was used to determine the kidney\'s expression of cleaved caspase-3, Bax, Bcl-2, nuclear factor kappa-B (NF-κB), and tumor necrosis factor-alpha (TNF-α). Kidney tissue damage score (KTDS) was assessed using the hematoxylin-eosin (H&E) staining method.
UNASSIGNED: Cisplatin significantly increased serum Cr, KTDS, MDA, BUN levels, NF-κB, TNF-α, cleaved caspase-3, and Bax protein expression in the cisplatin group compared to the control group (P < 0.01). Additionally, cisplatin significantly decreased the kidney tissue\'s TAC and GSH content, activity levels of SOD, catalase, and GPx indicators, and expression of Bcl-2 protein (P < 0.05). TCJ and carvacrol significantly ameliorated these indicators in the cisplatin + TCJ (150 mg/kg) and cisplatin + carvacrol (10 mg/kg) groups compared to the cisplatin group (P < 0.05).
UNASSIGNED: TCJ (150 mg/kg) and its main component, carvacrol, could somewhat reduce cisplatin-induced nephrotoxicity through their anti-inflammatory, antioxidant, and anti-apoptotic effects.
摘要：
■顺铂，抗癌化疗药物,有肾毒性作用。胸腺卡拉马尼（TCJ）由于其主要成分而具有抗氧化作用。
■在当前的研究中，我们评估了TCJ提取物及其主要化合物对顺铂诱导的小鼠肾毒性的影响.
■在研究中使用42只雄性小鼠。根据他们的团体，动物接受了生理盐水，香芹酚（10mg/kg），或TCJ提取物（50、100和150mg/kg）持续10天。第五天，小鼠接受顺铂（7.5mg/kg，i.p.）。10天后,测量血清肌酐（Cr）和血尿素氮（BUN）水平。此外,丙二醛（MDA）和谷胱甘肽（GSH）含量，以及超氧化物歧化酶（SOD）的活性水平，过氧化氢酶,和谷胱甘肽过氧化物酶（GPx），测定肾组织的总抗氧化能力(TAC)。蛋白质印迹法用于确定肾脏裂解的caspase-3，Bax的表达，Bcl-2,核因子κB(NF-κB),和肿瘤坏死因子-α(TNF-α)。使用苏木精-伊红(H&E)染色方法评估肾组织损伤评分(KTDS)。
■顺铂显著增加血清Cr,KTDS,MDA,BUN水平,NF-κB,TNF-α,顺铂组caspase-3、Bax蛋白表达明显低于对照组(P<0.01)。此外,顺铂显著降低肾组织的TAC和GSH含量，SOD的活性水平，过氧化氢酶,和GPx指标，Bcl-2蛋白的表达(P<0.05)。与顺铂组相比，TCJ和香芹酚在顺铂TCJ（150mg/kg）和顺铂香芹酚（10mg/kg）组中的这些指标显着改善（P<0.05）。
■TCJ（150mg/kg）及其主要成分，香芹酚,可以通过抗炎在某种程度上减少顺铂诱导的肾毒性，抗氧化剂，和抗凋亡作用。
■在当前的研究中，我们评估了TCJ提取物及其主要化合物对顺铂诱导的小鼠肾毒性的影响.
■在研究中使用42只雄性小鼠。根据他们的团体，动物接受了生理盐水，香芹酚（10mg/kg），或TCJ提取物（50、100和150mg/kg）持续10天。第五天，小鼠接受顺铂（7.5mg/kg，i.p.）。10天后,测量血清肌酐（Cr）和血尿素氮（BUN）水平。此外,丙二醛（MDA）和谷胱甘肽（GSH）含量，以及超氧化物歧化酶（SOD）的活性水平，过氧化氢酶,和谷胱甘肽过氧化物酶（GPx），测定肾组织的总抗氧化能力(TAC)。蛋白质印迹法用于确定肾脏裂解的caspase-3，Bax的表达，Bcl-2,核因子κB(NF-κB),和肿瘤坏死因子-α(TNF-α)。使用苏木精-伊红(H&E)染色方法评估肾组织损伤评分(KTDS)。
■顺铂显著增加血清Cr,KTDS,MDA,BUN水平,NF-κB,TNF-α,顺铂组caspase-3、Bax蛋白表达明显低于对照组(P<0.01)。此外,顺铂显著降低肾组织的TAC和GSH含量，SOD的活性水平，过氧化氢酶,和GPx指标，Bcl-2蛋白的表达(P<0.05)。与顺铂组相比，TCJ和香芹酚在顺铂TCJ（150mg/kg）和顺铂香芹酚（10mg/kg）组中的这些指标显着改善（P<0.05）。
■TCJ（150mg/kg）及其主要成分，香芹酚,可以通过抗炎在某种程度上减少顺铂诱导的肾毒性，抗氧化剂，和抗凋亡作用。
