PDF(Pubmed)
Abstract：
UNASSIGNED: The objective of this study was to investigate the cytological features and diagnostic significance of cerebrospinal fluid (CSF) in bacterial meningitis (BM).
UNASSIGNED: Patients diagnosed with BM at the First Affiliated Hospital of Nanchang University Hospital between August 2021 and April 2022 were enrolled. Clinical, cranial imaging, CSF-next-generation sequencing, CSF examination, and CSF cytology data were retrospectively analyzed. CSF cytology samples were prepared using a CSF cell pelletizer (precipitation method) and stained using the May-Grunwald-Glemsa (MGG) method. The χ2 test was employed to compare the positive rate of routine CSF count and CSF cytology.
UNASSIGNED: Eight patients (four males and four females), aged 41-67 years, were included. Among them, two patients had undergone brain surgery within the past 4 months, one patient had an 8-year history of otitis media, and two patients had a history of sudden toothache. Clinical manifestations included fever, headache, sudden disturbance of consciousness, and neck stiffness. CSF cytology revealed abnormal inflammatory changes dominated by neutrophils in seven patients. Routine CSF cell counts exceeded 100/uL in only four cases, indicating a higher positive rate of CSF cytology for detecting CSF inflammatory reactions compared to routine cell count.
UNASSIGNED: Comparative detection of bacteria through the observation of CSF cytology inflammatory status in BM patients are more useful for diagnosing BM than routine CSF counts.
UNASSIGNED: Patients diagnosed with BM at the First Affiliated Hospital of Nanchang University Hospital between August 2021 and April 2022 were enrolled. Clinical, cranial imaging, CSF-next-generation sequencing, CSF examination, and CSF cytology data were retrospectively analyzed. CSF cytology samples were prepared using a CSF cell pelletizer (precipitation method) and stained using the May-Grunwald-Glemsa (MGG) method. The χ2 test was employed to compare the positive rate of routine CSF count and CSF cytology.
UNASSIGNED: Eight patients (four males and four females), aged 41-67 years, were included. Among them, two patients had undergone brain surgery within the past 4 months, one patient had an 8-year history of otitis media, and two patients had a history of sudden toothache. Clinical manifestations included fever, headache, sudden disturbance of consciousness, and neck stiffness. CSF cytology revealed abnormal inflammatory changes dominated by neutrophils in seven patients. Routine CSF cell counts exceeded 100/uL in only four cases, indicating a higher positive rate of CSF cytology for detecting CSF inflammatory reactions compared to routine cell count.
UNASSIGNED: Comparative detection of bacteria through the observation of CSF cytology inflammatory status in BM patients are more useful for diagnosing BM than routine CSF counts.
摘要：
■这项研究的目的是研究脑脊液（CSF）在细菌性脑膜炎（BM）中的细胞学特征和诊断意义。
■纳入2021年8月至2022年4月在南昌大学医院第一附属医院诊断为BM的患者。临床,头颅成像,CSF-下一代测序，脑脊液检查,和脑脊液细胞学资料进行回顾性分析。使用CSF细胞造粒机(沉淀法)制备CSF细胞学样品,并使用May-Grunwald-Glemsa(MGG)方法染色。采用χ2检验比较常规脑脊液计数和脑脊液细胞学检查的阳性率。
■8名患者（4名男性和4名女性），41-67岁,包括在内。其中,在过去的4个月里，有两名患者接受了脑部手术，一名患者有8年中耳炎病史,两名患者有突发性牙痛的病史。临床表现包括发热，头痛,突然的意识障碍，颈部僵硬。脑脊液细胞学检查显示7例患者的中性粒细胞异常炎症变化。只有4例脑脊液细胞常规计数超过100/uL，表明与常规细胞计数相比，脑脊液细胞学检查检测脑脊液炎症反应的阳性率更高。
■通过观察BM患者的CSF细胞学炎症状态进行细菌的比较检测对于诊断BM比常规CSF计数更有用。
■纳入2021年8月至2022年4月在南昌大学医院第一附属医院诊断为BM的患者。临床,头颅成像,CSF-下一代测序，脑脊液检查,和脑脊液细胞学资料进行回顾性分析。使用CSF细胞造粒机(沉淀法)制备CSF细胞学样品,并使用May-Grunwald-Glemsa(MGG)方法染色。采用χ2检验比较常规脑脊液计数和脑脊液细胞学检查的阳性率。
■8名患者（4名男性和4名女性），41-67岁,包括在内。其中,在过去的4个月里，有两名患者接受了脑部手术，一名患者有8年中耳炎病史,两名患者有突发性牙痛的病史。临床表现包括发热，头痛,突然的意识障碍，颈部僵硬。脑脊液细胞学检查显示7例患者的中性粒细胞异常炎症变化。只有4例脑脊液细胞常规计数超过100/uL，表明与常规细胞计数相比，脑脊液细胞学检查检测脑脊液炎症反应的阳性率更高。
■通过观察BM患者的CSF细胞学炎症状态进行细菌的比较检测对于诊断BM比常规CSF计数更有用。
