Abstract：
OBJECTIVE: Intermittent catheters (ICs) are commonly used in bladder management, but catheter-associated urinary tract infections (CAUTIs) remain challenging. Insertion tips may reduce the risk of CAUTIs by minimizing bacterial transfer along the urinary tract. However, there are few laboratory tests to evaluate such technologies. We describe the use of an adapted in vitro urethra agar model to assess bacterial displacement by ICs.
RESULTS: Simulated urethra agar channels (UACs) were prepared with catheter-specific sized channels in selective media specific to the challenge organisms. UACs were inoculated with Escherichia coli and Enterococcus faecalis before insertion of ICs, and enumeration of UAC sections was performed following insertion. Four ICs were evaluated: Cure Catheter® Closed System (CCS), VaPro Plus Pocket™, Bard® Touchless® Plus, and SpeediCath® Flex Set. CCS demonstrated significantly reduced bacterial displacement along the UACs compared to the other ICs and was also the only IC with undetectable levels of bacteria toward the end of the UAC (representing the proximal urethra).
CONCLUSIONS: The bacterial displacement test demonstrated significant differences in bacterial transfer between the test ICs with insertion tips, which may reflect their different designs. This method is useful for evaluating CAUTI prevention technology and may help guide future technology innovations.
RESULTS: Simulated urethra agar channels (UACs) were prepared with catheter-specific sized channels in selective media specific to the challenge organisms. UACs were inoculated with Escherichia coli and Enterococcus faecalis before insertion of ICs, and enumeration of UAC sections was performed following insertion. Four ICs were evaluated: Cure Catheter® Closed System (CCS), VaPro Plus Pocket™, Bard® Touchless® Plus, and SpeediCath® Flex Set. CCS demonstrated significantly reduced bacterial displacement along the UACs compared to the other ICs and was also the only IC with undetectable levels of bacteria toward the end of the UAC (representing the proximal urethra).
CONCLUSIONS: The bacterial displacement test demonstrated significant differences in bacterial transfer between the test ICs with insertion tips, which may reflect their different designs. This method is useful for evaluating CAUTI prevention technology and may help guide future technology innovations.
摘要：
目的：间歇性导尿管（IC）常用于膀胱管理，但导尿管相关尿路感染（CAUTIs）仍然具有挑战性。插入尖端可以通过最小化沿泌尿道的细菌转移来降低CAUTI的风险。然而,很少有实验室测试来评估这种技术。我们描述了使用适应的体外尿道琼脂模型来评估IC的细菌置换。
结果：模拟尿道琼脂通道（UAC）是在对攻击生物具有特异性的选择性培养基中用导管特异性大小的通道制备的。在插入IC之前用大肠杆菌和粪肠杆菌接种UAC,并且在插入之后进行UAC切片的计数。评估了四个IC:CureCatheter®封闭系统(CCS),VaProPlusPocketTM,Bard®Touchless®Plus,和SpeediCath®Flex套装。与其他IC相比，CCS显示出沿着UAC的细菌位移显着降低，并且也是唯一在UAC末端（代表近端尿道）具有无法检测到的细菌水平的IC。
结论：细菌置换试验表明，具有插入尖端的测试IC之间的细菌转移存在显着差异，这可能反映了它们的不同设计。该方法可用于评估CAUTI预防技术，并有助于指导未来的技术创新。
结果：模拟尿道琼脂通道（UAC）是在对攻击生物具有特异性的选择性培养基中用导管特异性大小的通道制备的。在插入IC之前用大肠杆菌和粪肠杆菌接种UAC,并且在插入之后进行UAC切片的计数。评估了四个IC:CureCatheter®封闭系统(CCS),VaProPlusPocketTM,Bard®Touchless®Plus,和SpeediCath®Flex套装。与其他IC相比，CCS显示出沿着UAC的细菌位移显着降低，并且也是唯一在UAC末端（代表近端尿道）具有无法检测到的细菌水平的IC。
结论：细菌置换试验表明，具有插入尖端的测试IC之间的细菌转移存在显着差异，这可能反映了它们的不同设计。该方法可用于评估CAUTI预防技术，并有助于指导未来的技术创新。
