Abstract：
The last several decades have witnessed the success and popularity of colorimetric lateral flow assay (CLFA) in point-of-care testing. Driven by increasing demand, great efforts have been directed toward enhancing the detection sensitivity of CLFA. Recently, platinum-group metal nanoparticles (PGM NPs) with peroxidase-like activities have emerged as a type of promising colorimetric labels for enhancing the sensitivity of CLFA. By incorporating a simple and rapid post-treatment process, the PGM NP-based CLFAs are orders of magnitude more sensitive than conventional gold nanoparticle-based CLFAs. In this perspective, the study begins with introducing the design, synthesis, and characterization of PGM NPs with peroxidase-like activities. The current techniques for surface modification of PGM NPs are then discussed, followed by operation and optimization of PGM NP-based CLFAs. Afterward, opinions are provided on the social impact of PGM NP-based CLFAs. Lastly, this perspective is concluded with an outlook of future research directions in this emerging field, where the challenges and opportunities are discussed.
摘要：
在过去的几十年中，比色侧流测定（CLFA）在即时测试中取得了成功和普及。在需求增加的推动下，已经为提高CLFA的检测灵敏度做出了巨大的努力。最近,具有过氧化物酶样活性的铂族金属纳米颗粒（PGMNP）已成为一种有前途的比色标记，可增强CLFA的灵敏度。通过加入简单快速的后处理过程，基于PGMNP的CLFA比常规的基于金纳米颗粒的CLFA更敏感。从这个角度来看,这项研究从介绍设计开始，合成，具有过氧化物酶样活性的PGMNP的表征。然后讨论了PGMNP表面改性的当前技术，其次是基于PGMNP的CLFA的操作和优化。之后,对基于PGMNP的CLFA的社会影响提供了意见。最后,这一观点是对这一新兴领域未来研究方向的展望，讨论挑战和机遇。
