METHODS: Formalin-fixed paraffin-embedded (FFPE) blocks of epithelial salivary gland neoplasms were retrieved from the archives of the Oral and Maxillofacial Pathology Department, Faculty of Dentistry, Cairo University within the 5-year period from 2016 to 2020. Immunohistochemistry was used to assess HCV core antigen, while reverse transcription polymerase chain reaction was employed for the evaluation of HCV RNA.
RESULTS: A total of 44 specimens were collected, 28 of which were benign neoplasms and 16 were malignant neoplasms. There was a statistically significant difference in HCV positivity between the two groups (P-value = 0.036). Benign tumors showed a statistically significant lower percentage of positive cases than malignant tumors. The localization of staining was also evaluated, revealing various patterns of HCV core antigen expression, including diffuse cytoplasmic, patchy cytoplasmic, nuclear, and a combination of nuclear and cytoplasmic expression. There was no statistically significant difference between the expression patterns in benign and malignant tumors (P-value = 0.616). Given that Pleomorphic Adenoma and Mucoepidermoid Carcinoma were the predominant tumor types in this study, four cases were selected for RNA detection. HCV RNA was detected in all cases using RT-PCR.
CONCLUSIONS: HCV core antigen is frequently detected in SGNs and is suggested to be a potential risk factor for the development of these neoplasms. Further studies are required to discover other biomarkers, their roles, and the pathways associated with HCV in SGNs.
方法:从口腔颌面病理科档案中检索福尔马林固定石蜡包埋(FFPE)的上皮唾液腺肿瘤块,牙科学院,开罗大学在2016年至2020年的5年期间。免疫组织化学用于评估HCV核心抗原,而逆转录聚合酶链反应用于评估HCVRNA。
结果:共收集了44个标本,其中良性肿瘤28例,恶性肿瘤16例。两组之间的HCV阳性有统计学意义(P值=0.036)。与恶性肿瘤相比,良性肿瘤显示出统计学上显着的阳性病例百分比较低。还评估了染色的定位,揭示HCV核心抗原表达的各种模式,包括弥漫性细胞质,斑片状细胞质,核,以及细胞核和细胞质表达的组合。在良性和恶性肿瘤中的表达模式之间没有统计学上的显着差异(P值=0.616)。鉴于多形性腺瘤和粘液表皮样癌是本研究的主要肿瘤类型,选择4例进行RNA检测。所有病例均采用RT-PCR检测HCVRNA。
结论:HCV核心抗原经常在SGN中检测到,被认为是这些肿瘤发展的潜在危险因素。需要进一步的研究来发现其他生物标志物,他们的角色,以及SGN中与HCV相关的途径。