PDF(Pubmed)
Abstract：
Identification of the sex of modern, fossil and archaeological animal remains offers many insights into their demography, mortality profiles and domestication pathways. However, due to many-factors, sex determination of osteological remains is often problematic. To overcome this, we have developed an innovative protocol to determine an animal\'s sex from tooth enamel, by applying label-free quantification (LFQ) of two unique AmelY peptides \'LRYPYP\' (AmelY;[M+2] 2 + 404.7212 m/z) and \'LRYPYPSY\' (AmelY;[M+2] 2 + 529.7689 m/z) that are only present in the enamel of males. We applied this method to eight modern cattle (Bos taurus) of known sex, and correctly assigned them to sex. We then applied the same protocol to twelve archaeological Bos teeth from the Neolithic site of Beisamoun, Israel (8-th-7-th millennium BC) and determined the sex of the archaeological samples. Since teeth are usually better preserved than bones, this innovative protocol has potential to facilitate sex determination in ancient and modern bovine remains that currently cannot be sexed.
摘要：
现代性别的鉴定，化石和考古动物遗骸提供了许多有关其人口统计的见解，死亡率和驯化途径。然而,由于多种因素,骨性遗骸的性别确定通常是有问题的。为了克服这一点，我们已经开发了一个创新的协议，以确定一个动物的性别，从牙釉质，通过对两种仅存在于雄性釉质中的独特AmelY肽\'LRYPYP\'(AmelY;[M+2]2+404.7212m/z)和\'LRYPYPSY\'(AmelY;[M+2]2+529.7689m/z)进行无标记定量(LFQ)。我们将这种方法应用于已知性别的八头现代牛（Bostaurus），并正确地将他们分配给性爱。然后，我们将相同的协议应用于来自Beisamoun新石器时代遗址的十二个考古Bos牙齿，以色列（公元前8-7千年）并确定了考古样本的性别。因为牙齿通常比骨头保存得更好，这种创新的协议有可能促进目前无法进行性别鉴定的古代和现代牛遗骸的性别鉴定。
