Abstract：
Personalized three-dimensional (3D) printed foods rich in probiotics were investigated. Lactiplantibacillus plantarum (Lp), as a representative of probiotics, was used to investigate the 3D printing of probiotic-rich dysphagia foods. Here, whey protein isolate nanofibrils (WPNFs) were coated and anchored on bacterial surfaces via biointerfacial supramolecular self-assembly, providing protection against environmental stress and the 3D printing process. The optimized composite gels consisting of High acyl gellan gum (0.25 g), whey protein isolate (1.25 g), fructooligosaccharides (0.75 g), Lp-WPNFs-Glyceryl tributyrate emulsion (Φ = 40%, 3.75 mL) can realize 3D printing, and exhibit high resolution, and stable shape. The viable cell count is higher than 8.0 log CFU/g. They are particularly suitable for people with dysphagia and are classified as level 5-minced & moist in the international dysphagia diet standardization initiative framework. The results provide new insights into the development of WPNFs-coating on bacterial surfaces to deliver probiotics and 3D printed food rich in probiotics.
摘要：
研究了富含益生菌的个性化三维（3D）打印食品。植物乳杆菌(Lp)，作为益生菌的代表，用于研究富含益生菌的吞咽困难食品的3D打印。这里,乳清蛋白分离物纳米原纤维（WPNFs）通过生物界面超分子自组装包被并锚定在细菌表面，提供对环境压力和3D打印过程的保护。优化的复合凝胶由高酰基结冷胶（0.25g）组成，乳清分离蛋白（1.25g），低聚果糖（0.75g），Lp-WPNFs-三丁酸甘油酯乳剂(Φ=40%，3.75mL）可实现3D打印，并表现出高分辨率，形状稳定。活细胞计数高于8.0logCFU/g。它们特别适用于吞咽困难的人，在国际吞咽困难饮食标准化倡议框架中被归类为5级切碎和湿润。这些结果为细菌表面上的WPNFs涂层的开发提供了新的见解，以提供益生菌和富含益生菌的3D打印食品。
