Abstract：
Microbial metabolism in landfill leachate systems is critically important in driving the degradation reactions of organic pollutants, including the emerging pollutant bisphenol A (BPA). However, little research has addressed the microbial degradation of BPA in landfill leachate and its interactions with nitrogen (N), sulfur (S), and methane (CH4) metabolism on a global scale. To this end, in this study on a global scale, an extremely high concentration of BPA was detected throughout the global landfill leachates. Subsequent reconstructive analyses of metagenomic datasets from 113 sites worldwide revealed that the predominant BPA-degrading microflora included Proteobacteria, Firmicutes, and Bacteroidota. Further metabolic analyses revealed that all four biochemical pathways involved in the degradation of BPA were achieved through biochemical cooperation between different bacterial members of the community. In addition, BPA degraders have also been found to actively collaborate synergistically with non-BPA degraders in the N and S removal as well as CH4 catabolism in landfill leachates. Collectively, this study not only provides insights into the dominant microbial communities and specific types of BPA-degrading microbial members in the community of landfill leachates worldwide, but also reveals the synergistic interactions between BPA mineralization and N, S, and CH4 metabolism. These findings offer valuable and important insights for future comprehensive and in-depth investigations into BPA metabolism in different environments.
摘要：
垃圾渗滤液系统中的微生物代谢对于驱动有机污染物的降解反应至关重要。包括新兴污染物双酚A(BPA)。然而,很少有研究解决垃圾渗滤液中BPA的微生物降解及其与氮（N）的相互作用，硫(S),甲烷（CH4）代谢在全球范围内。为此,在全球范围内的这项研究中，在全球垃圾渗滤液中检测到极高浓度的BPA。随后对来自全球113个地点的宏基因组数据集进行的重建分析显示，主要的降解BPA的微生物群包括变形杆菌，Firmicutes,和拟杆菌。进一步的代谢分析显示，参与BPA降解的所有四种生化途径都是通过社区不同细菌成员之间的生化合作实现的。此外,还发现BPA降解剂与非BPA降解剂在N和S去除以及垃圾渗滤液中的CH4分解代谢方面积极协同协作。总的来说，这项研究不仅提供了对全球垃圾渗滤液群落中主要微生物群落和特定类型的BPA降解微生物成员的见解，而且还揭示了BPA矿化与N之间的协同相互作用，S,和CH4代谢。这些发现为未来对不同环境中BPA代谢的全面和深入的研究提供了有价值和重要的见解。
