PDF(Pubmed)
Abstract：
To investigate the sex-dependent differentiation of Sox10 cells and their response to pathological conditions such as lipopolysaccharide (LPS) exposure or ischemia, we utilized Sox10 Cre-ERT2, tdTomato mice. Tamoxifen administration induced the expression of red fluorescent protein (RFP) in these cells, facilitating their subsequent tracking and analysis after LPS injection and ischemia via immunofluorescence staining. Propidium iodide (PI) was injected to label necrotic cells following LPS administration. We found that the conversion of Sox10 cells to pericytes in female mice was significantly higher than in male mice, especially in those exposed to LPS. After LPS injection, the number of PI+ necrotic cells were significantly greater in females than in males. Moreover, RFP+ cells did not co-localize with glial fibrillary acidic protein (GFAP) or cluster of differentiation 11b (CD11b). Similarly, after brain ischemia, RFP+ cells did not express cluster of differentiation 13 (CD13), neuronal nuclei (NeuN), GFAP, or ionised calcium binding adaptor molecule 1 (Iba-1). These findings indicate that the conversion of Sox10 cells to pericytes following LPS exposure is sex-dependent, with neither male nor female groups showing differentiation into other cell types after LPS exposure or under ischemic conditions. The differences in LPS-induced necrosis of pericytes between sexes may explain the variations in the conversion of Sox10 cells to pericytes in both sexes.
摘要：
探讨Sox10细胞的性别依赖性分化及其对脂多糖(LPS)暴露或缺血等病理条件的反应。我们使用Sox10Cre-ERT2，td番茄小鼠。他莫昔芬给药诱导这些细胞中红色荧光蛋白（RFP）的表达，通过免疫荧光染色促进他们在LPS注射和缺血后的后续跟踪和分析。在LPS施用后,注射碘化丙啶(PI)以标记坏死细胞。我们发现雌性小鼠Sox10细胞向周细胞的转化率明显高于雄性小鼠,尤其是那些暴露于LPS的人。注射LPS后,女性的PI+坏死细胞数量明显多于男性。此外，RFP+细胞不与神经胶质原纤维酸性蛋白(GFAP)或分化簇11b(CD11b)共定位。同样，脑缺血后，RFP+细胞不表达分化簇13（CD13），神经元核（NeuN），GFAP,或电离的钙结合衔接分子1(Iba-1)。这些发现表明，LPS暴露后Sox10细胞向周细胞的转化是性别依赖性的，在LPS暴露后或在缺血条件下，雄性和雌性组均未显示分化为其他细胞类型。两性之间LPS诱导的周细胞坏死的差异可以解释两性Sox10细胞向周细胞转化的变化。
