Abstract：
Adriamycin is a cytotoxic anthracycline antibiotic used to treat a wide variety of cancers. This study was made to detect the possible prophylactic effects of combining garlic and resveratrol in preventing adriamycin-induced pulmonary cytotoxicity. This study was conducted on a total number of 60 adult male albino rats. The rats were divided in an equally random manner into 6 groups: group I rats received nothing, group II received a dose of 50 mg/kg garlic extract orally for 3 weeks, group III received resveratrol in a dose of 20 mg/kg/day orally for 3 weeks, group IV rats were injected with 20 mg/kg adriamycin as a single dose via intraperitoneal route, group V received garlic extract for 3 weeks, then were injected with adriamycin in the same stated doses, and Group VI received garlic extract and resveratrol in same stated dose for 3 weeks, then were injected with adriamycin in the same stated dose. Lung specimens were processed for light microscopic, ultrastructural, and immunohistochemical studies. Adriamycin treatment caused histological alterations, thicker interstitial septa, extensive cellular infiltration, hypertrophied arterial wall, marked inducible Nitric Oxide Synthase immunoreaction, type I pneumocytes with destructed organelles as well as type II pneumocytes having large vacuoles. The combined garlic and resveratrol group demonstrated a considerable improvement in the changes to the histology and ultrastructure of adriamycin-induced lung injury. Combining garlic and resveratrol can prevent adriamycin-induced lung cytotoxicity in albino rats.
摘要：
阿霉素是用于治疗多种癌症的细胞毒性蒽环类抗生素。本研究旨在检测大蒜和白藜芦醇联合预防阿霉素诱导的肺细胞毒性的可能作用。这项研究是对60只成年雄性白化病大鼠进行的。将大鼠随机平均分为6组：第I组大鼠未接受任何药物，II组口服50mg/kg大蒜提取物，持续3周，第三组口服白藜芦醇20mg/kg/天，持续3周，IV组大鼠腹腔注射20mg/kg阿霉素单剂量,V组接受大蒜提取物3周，然后以相同的剂量注射阿霉素，第VI组接受相同剂量的大蒜提取物和白藜芦醇3周，然后注射相同剂量的阿霉素。肺标本进行光学显微镜处理，超微结构，和免疫组织化学研究。阿霉素治疗引起组织学改变，较厚的间隙隔片，广泛的细胞浸润，肥大的动脉壁，标记的诱导型一氧化氮合酶免疫反应，具有破坏的细胞器的I型肺细胞以及具有大液泡的II型肺细胞。大蒜和白藜芦醇联合组对阿霉素引起的肺损伤的组织学和超微结构的改变有了显着改善。大蒜和白藜芦醇联合使用可以预防阿霉素诱导的白化病大鼠肺细胞毒性。
