Abstract：
Human mesenchymal stromal cells (MSCs) have gained significant interest as cell-based therapeutics for organ restoration in the field of regenerative medicine. More recently, substantial attention has been directed toward cell-free therapy, achieved through the utilization of soluble factors possessing trophic and immunomodulatory properties present in the MSC secretome. This collection of soluble factors can be found either freely in the secretome or packed within its vesicular fraction, known as extracellular vesicles (EVs). MSCs can be derived from various tissue sources, each involving different extraction methods and yielding varying cell amounts. In this study, we describe a nonenzymatic procedure for a straightforward isolation of MSCs from the fetal dermis and the adult dermis. The results demonstrate the isolation of a cell population with a uniform MSC immunophenotype from the earliest passages (approximately 90% positive for the classical MSC markers CD90, CD105, and CD73, while negative for the hematopoietic markers CD34 and CD45, as well as HLA-DR). Additionally, we describe the procedures for cell expansion, banking, and secretome collection.
摘要：
在再生医学领域中,人类间充质基质细胞(MSC)作为用于器官修复的基于细胞的治疗剂已经获得了显著的兴趣。最近,大量的注意力已经转向无细胞治疗，通过利用MSC分泌组中具有营养和免疫调节特性的可溶性因子来实现。这种可溶性因子的集合可以自由地存在于分泌组中或包装在其囊泡部分中，称为细胞外囊泡(EV)。MSCs可以来源于各种组织来源，每个涉及不同的提取方法和产生不同的细胞量。在这项研究中,我们描述了一种从胎儿真皮和成人真皮中直接分离MSCs的非酶方法.结果证明从最早的传代(对于经典MSC标志物CD90、CD105和CD73约90%阳性,而对于造血标志物CD34和CD45以及HLA-DR阴性)分离出具有均匀MSC免疫表型的细胞群。此外,我们描述了细胞扩增的程序，banking,和秘密收集。
