Abstract：
A smartphone-assisted portable dual-mode immunoassay was constructed based on curcumin nanoparticles (CNPs) and carbon dots (CDs) for gentamicin (GEN) detection. CNPs were labeled with goat anti-mouse IgG (Ab2) to create a conjugation that coupled dual signals to concentrations of GEN antigens. CNPs were introduced to pH 7.4 water and showed insignificant color and optical responses. When exposed to the high pH environment, the structure of CNPs changed and color and optical properties were restored. Because of the inner filter effect (IFE) between CNPs and CDs, the fluorescence of CNPs at 550 nm quenched the fluorescence of CDs at 450 nm. Colorimetry and ratiometric fluorescence (F550 nm/F450 nm) dual-mode immunoassay linearly correlated with GEN ranged from 10-4 to 100 µg/mL with a detection limit (LOD) of 8.98 × 10-5 µg/mL and 4.66 × 10-5 µg/mL, respectively. This work supplied a portable, sensitive, and specific platform to detect GEN.
摘要：
基于姜黄素纳米颗粒（CNP）和碳点（CD）构建了智能手机辅助的便携式双模式免疫测定法，用于庆大霉素（GEN）检测。用山羊抗小鼠IgG(Ab2)标记CNP以产生将双信号与浓度的GEN抗原偶联的缀合。将CNP引入pH7.4的水中,并显示不显著的颜色和光学响应。当暴露于高pH环境时，CNPs的结构发生变化，颜色和光学性质得到恢复。由于CNP和CD之间的内部滤波效应（IFE），CNP在550nm处的荧光猝灭CD在450nm处的荧光。比色法和比率荧光（F550nm/F450nm）双模式免疫测定与GEN线性相关，范围为10-4至100µg/mL，检出限（LOD）为8.98×10-5µg/mL和4.66×10-5µg/mL，分别。这项工作提供了一个便携式，敏感,和特定的平台来检测GEN。
