UNASSIGNED: This article provides an update on in vitro transporter assays, focusing on new uses of transfected cells, time-dependent inhibition, transporter induction, and complex model systems.
UNASSIGNED: The newer in vitro assays add to the toolbox in defining new drugs as transporter substrates, inhibitors, or inducers. Complex models such as spheroids, organoids, and microphysiological systems require standardization and further research with model transporter substrates and inhibitors. In drug discovery, the more traditional transporter assays may be employed as substrate and inhibitor screening assays. In drug development, more complex cell models can be employed in later drug development to better understand how transporter(s) are involved in the absorption, distribution, and excretion of new drugs.
■本文提供了有关体外转运蛋白测定的最新信息,专注于转染细胞的新用途,时间依赖性抑制,转运蛋白诱导,和复杂的模型系统。
■较新的体外测定法在定义新药作为转运底物时增加了工具箱,抑制剂,或诱导物。复杂的模型,如球体,类器官,和微生理系统需要标准化和进一步研究模型转运蛋白底物和抑制剂。在药物发现中,更传统的转运蛋白测定可以用作底物和抑制剂筛选测定。在药物开发中,更复杂的细胞模型可以在以后的药物开发中使用,以更好地了解转运蛋白如何参与吸收,分布,以及新药的排泄.