PDF(Pubmed)
Abstract：
UNASSIGNED: The proximity of activated T cells and mast cells in the lesional skin of patients with chronic spontaneous urticaria (CSU) is held to contribute to the development of wheals and angioedema. In a previous study, we demonstrated that increased IL-17 expression in T cells and mast cells in skin lesions of patients with CSU is associated with T/mast cell proximity, but the mechanisms that drive T cell/mast cell co-localization remain unknown.
UNASSIGNED: To assess if chemokines expressed in lesional CSU skin contribute to T cell/mast cell proximity.
UNASSIGNED: Biopsies from lesional CSU skin were compared to biopsies from healthy skin for expression of CCR5 and its ligand CCL3 by CD4+ T cells and mast cells, respectively.
UNASSIGNED: Numbers of CCR5-positive CD4+ T cells in lesional CSU skin were significantly increased as compared to healthy normal skin (p < 0.0001). The number of mast cells expressing CCL3 (ligand for CCR5) in CSU skin was also increased (p < 0.0002) and significant association with T-cell close proximity (p < 0.0001) is noticed.
UNASSIGNED: The close proximity of T cells and mast cells in the skin of severe CSU may be driven, at least in part by increased CCR5 and CCL3 expression. Therapies that target CCL3 interaction with CCR5 should be assessed for their effects in CSU.
UNASSIGNED: To assess if chemokines expressed in lesional CSU skin contribute to T cell/mast cell proximity.
UNASSIGNED: Biopsies from lesional CSU skin were compared to biopsies from healthy skin for expression of CCR5 and its ligand CCL3 by CD4+ T cells and mast cells, respectively.
UNASSIGNED: Numbers of CCR5-positive CD4+ T cells in lesional CSU skin were significantly increased as compared to healthy normal skin (p < 0.0001). The number of mast cells expressing CCL3 (ligand for CCR5) in CSU skin was also increased (p < 0.0002) and significant association with T-cell close proximity (p < 0.0001) is noticed.
UNASSIGNED: The close proximity of T cells and mast cells in the skin of severe CSU may be driven, at least in part by increased CCR5 and CCL3 expression. Therapies that target CCL3 interaction with CCR5 should be assessed for their effects in CSU.
摘要：
■慢性自发性荨麻疹（CSU）患者的病变皮肤中活化的T细胞和肥大细胞的接近被认为有助于风团和血管性水肿的发展。在之前的研究中,我们证明了CSU患者皮肤病变中T细胞和肥大细胞中IL-17表达的增加与T/肥大细胞接近相关,但是驱动T细胞/肥大细胞共定位的机制仍然未知。
■评估病变CSU皮肤中表达的趋化因子是否有助于T细胞/肥大细胞接近。
■将病变CSU皮肤的活检与健康皮肤的活检进行比较，以确定CD4T细胞和肥大细胞表达CCR5及其配体CCL3，分别。
■病灶CSU皮肤中CCR5阳性CD4+T细胞的数量与健康正常皮肤相比显著增加(p<0.0001)。CSU皮肤中表达CCL3(CCR5的配体)的肥大细胞的数量也增加(p<0.0002),并且注意到与T细胞紧密接近的显著关联(p<0.0001)。
■严重CSU的皮肤中T细胞和肥大细胞的紧密接近可能被驱动，至少部分通过增加CCR5和CCL3表达。应评估针对CCL3与CCR5相互作用的疗法在CSU中的效果。
■评估病变CSU皮肤中表达的趋化因子是否有助于T细胞/肥大细胞接近。
■将病变CSU皮肤的活检与健康皮肤的活检进行比较，以确定CD4T细胞和肥大细胞表达CCR5及其配体CCL3，分别。
■病灶CSU皮肤中CCR5阳性CD4+T细胞的数量与健康正常皮肤相比显著增加(p<0.0001)。CSU皮肤中表达CCL3(CCR5的配体)的肥大细胞的数量也增加(p<0.0002),并且注意到与T细胞紧密接近的显著关联(p<0.0001)。
■严重CSU的皮肤中T细胞和肥大细胞的紧密接近可能被驱动，至少部分通过增加CCR5和CCL3表达。应评估针对CCL3与CCR5相互作用的疗法在CSU中的效果。
