PDF(Pubmed)
Abstract：
General anesthesia can impact a patient\'s memory and cognition by influencing hippocampal function. The CA1 and dentate gyrus (DG), serving as the primary efferent and gateway of the hippocampal trisynaptic circuit facilitating cognitive learning and memory functions, exhibit significant differences in cellular composition, molecular makeup, and responses to various stimuli. However, the effects of isoflurane-induced general anesthesia on CA1 and DG neuronal activity in mice are not well understood. In this study, utilizing electrophysiological recordings, we examined neuronal population dynamics and single-unit activity (SUA) of CA1 and DG in freely behaving mice during natural sleep and general anesthesia. Our findings reveal that isoflurane anesthesia shifts local field potential (LFP) to delta frequency and reduces the firing rate of SUA in both CA1 and DG, compared to wakefulness. Additionally, the firing rates of DG neurons are significantly lower than CA1 neurons during isoflurane anesthesia, and the recovery of theta power is slower in DG than in CA1 during the transition from anesthesia to wakefulness, indicating a stronger and more prolonged impact of isoflurane anesthesia on DG. This work presents a suitable approach for studying brain activities during general anesthesia and provides evidence for distinct effects of isoflurane anesthesia on hippocampal subregions.
摘要：
全身麻醉可通过影响海马功能影响患者的记忆和认知。CA1和齿状回（DG），作为促进认知学习和记忆功能的海马三突触回路的主要传出和网关，表现出细胞组成的显著差异，分子化妆，以及对各种刺激的反应。然而,异氟烷诱导的全身麻醉对小鼠CA1和DG神经元活性的影响尚不清楚.在这项研究中,利用电生理记录，我们检查了在自然睡眠和全身麻醉期间表现自由的小鼠的神经元种群动态和CA1和DG的单单位活动（SUA）。我们的发现表明，异氟醚麻醉将局部场电位（LFP）转移到δ频率，并降低了CA1和DG中SUA的放电率。与清醒相比。此外,在异氟烷麻醉期间，DG神经元的放电率显著低于CA1神经元，在从麻醉到清醒的过渡过程中，DG的θ功率恢复比CA1慢，表明异氟烷麻醉对DG的影响更强和更长时间。这项工作为研究全身麻醉期间的大脑活动提供了一种合适的方法，并为异氟烷麻醉对海马亚区的不同影响提供了证据。
