PDF(Pubmed)
Abstract：
BACKGROUND: Aging is a multifaceted process that affects all organ systems. With the increasing trend of population aging, aging-related diseases have resulted in significant medical challenges and socioeconomic burdens. Mesenchymal stromal cells (MSCs), due to their antioxidative stress, immunoregulatory, and tissue repair capabilities, hold promise as a potential anti-aging intervention.
METHODS: In this study, we transplanted MSCs into naturally aged rats at 24 months, and subsequently examined levels of aging-related factors such as β-galactosidase, superoxide dismutase, p16, p21 and malondialdehyde in multiple organs. Additionally, we assessed various aging-related phenotypes in these aged rats, including immune senescence, lipid deposition, myocardial fibrosis, and tissue damage. We also conducted a 16 S ribosomal ribonucleic acid (rRNA) analysis to study the composition of gut microbiota.
RESULTS: The results indicated that MSCs significantly reduced the levels of aging-associated and oxidative stress-related factors in multiple organs such as the heart, liver, and lungs of naturally aging rats. Furthermore, they mitigated chronic tissue damage and inflammation caused by aging, reduced levels of liver lipid deposition and myocardial fibrosis, alleviated aging-associated immunodeficiency and immune cell apoptosis, and positively influenced the gut microbiota composition towards a more youthful state. This research underscores the diverse anti-aging effects of MSCs, including oxidative stress reduction, tissue repair, metabolic regulation, and improvement of immune functions, shedding light on the underlying anti-aging mechanisms associated with MSCs.
CONCLUSIONS: The study confirms that MSCs hold great promise as a potential anti-aging approach, offering the possibility of extending lifespan and improving the quality of life in the elderly population.
METHODS: In this study, we transplanted MSCs into naturally aged rats at 24 months, and subsequently examined levels of aging-related factors such as β-galactosidase, superoxide dismutase, p16, p21 and malondialdehyde in multiple organs. Additionally, we assessed various aging-related phenotypes in these aged rats, including immune senescence, lipid deposition, myocardial fibrosis, and tissue damage. We also conducted a 16 S ribosomal ribonucleic acid (rRNA) analysis to study the composition of gut microbiota.
RESULTS: The results indicated that MSCs significantly reduced the levels of aging-associated and oxidative stress-related factors in multiple organs such as the heart, liver, and lungs of naturally aging rats. Furthermore, they mitigated chronic tissue damage and inflammation caused by aging, reduced levels of liver lipid deposition and myocardial fibrosis, alleviated aging-associated immunodeficiency and immune cell apoptosis, and positively influenced the gut microbiota composition towards a more youthful state. This research underscores the diverse anti-aging effects of MSCs, including oxidative stress reduction, tissue repair, metabolic regulation, and improvement of immune functions, shedding light on the underlying anti-aging mechanisms associated with MSCs.
CONCLUSIONS: The study confirms that MSCs hold great promise as a potential anti-aging approach, offering the possibility of extending lifespan and improving the quality of life in the elderly population.
摘要：
背景：衰老是影响所有器官系统的多方面过程。随着人口老龄化趋势的加剧,与衰老相关的疾病导致了重大的医疗挑战和社会经济负担。间充质基质细胞(MSCs),由于它们的抗氧化压力，免疫调节,和组织修复能力，作为一种潜在的抗衰老干预措施。
方法：在本研究中，我们在24个月时将MSCs移植到自然衰老的大鼠中，随后检查了衰老相关因子的水平，如β-半乳糖苷酶，超氧化物歧化酶,p16，p21和丙二醛在多个器官。此外,我们评估了这些老年大鼠的各种衰老相关表型，包括免疫衰老,脂质沉积，心肌纤维化，和组织损伤。我们还进行了16S核糖体核糖核酸（rRNA）分析，以研究肠道微生物群的组成。
结果：结果表明，MSCs可显著降低心脏等多器官中衰老相关因子和氧化应激相关因子的水平，肝脏,和自然衰老大鼠的肺。此外，它们减轻了老化引起的慢性组织损伤和炎症，肝脏脂质沉积和心肌纤维化水平降低，减轻衰老相关免疫缺陷和免疫细胞凋亡，并积极影响肠道微生物群组成，使其走向更年轻的状态。这项研究强调了MSCs的多种抗衰老作用，包括减少氧化应激，组织修复,代谢调节，和免疫功能的改善，揭示与MSCs相关的潜在抗衰老机制。
结论：该研究证实，MSCs作为一种潜在的抗衰老方法具有很大的前景。提供了延长寿命和提高老年人口生活质量的可能性。
方法：在本研究中，我们在24个月时将MSCs移植到自然衰老的大鼠中，随后检查了衰老相关因子的水平，如β-半乳糖苷酶，超氧化物歧化酶,p16，p21和丙二醛在多个器官。此外,我们评估了这些老年大鼠的各种衰老相关表型，包括免疫衰老,脂质沉积，心肌纤维化，和组织损伤。我们还进行了16S核糖体核糖核酸（rRNA）分析，以研究肠道微生物群的组成。
结果：结果表明，MSCs可显著降低心脏等多器官中衰老相关因子和氧化应激相关因子的水平，肝脏,和自然衰老大鼠的肺。此外，它们减轻了老化引起的慢性组织损伤和炎症，肝脏脂质沉积和心肌纤维化水平降低，减轻衰老相关免疫缺陷和免疫细胞凋亡，并积极影响肠道微生物群组成，使其走向更年轻的状态。这项研究强调了MSCs的多种抗衰老作用，包括减少氧化应激，组织修复,代谢调节，和免疫功能的改善，揭示与MSCs相关的潜在抗衰老机制。
结论：该研究证实，MSCs作为一种潜在的抗衰老方法具有很大的前景。提供了延长寿命和提高老年人口生活质量的可能性。
