Abstract：
In this work, a benzofuranone-derived fluorescent probe BFSF was developed for imaging the sulphite level in living hypoxia pulmonary cells. Under the excitation of 510 nm, BFSF showed a strong fluorescence response at 570 nm when reacted with sulphite. In the solution system, the constructed hypercapnia and serious hypercapnia conditions did not affect the fluorescence response. In comparison with the recently reported probes, BFSF suggested the advantages including rapid response, steady signal reporting, high specificity and low cytotoxicity upon living lung cells. Under a normal incubation atmosphere, BFSF realized the imaging of both exogenous and endogenous sulphite in living pulmonary cells. In particular, BFSF achieved imaging the decrease of the sulphite level under severe hypoxia as well as the recovery of the sulphite level with urgent oxygen supplement. With the imaging capability for the sulphite level in living pulmonary cells under hypoxia conditions, BFSF together with the information herein was meaningful for investigating the anaesthesia-related biological indexes.
摘要：
在这项工作中,开发了苯并呋喃酮衍生的荧光探针BFSF，用于对生活低氧肺细胞中的亚硫酸盐水平进行成像。在510nm的激发下，当与亚硫酸盐反应时,BFSF在570nm处显示强荧光响应。在解决方案系统中，构建的高碳酸血症和严重的高碳酸血症条件不影响荧光反应。与最近报道的探测器相比，BFSF提出了包括快速反应在内的优势，稳定的信号报告，对活肺细胞的高特异性和低细胞毒性。在正常的孵化气氛下，BFSF实现了活体肺细胞中外源性和内源性亚硫酸盐的成像。特别是,BFSF实现了在严重缺氧下亚硫酸盐水平的降低以及通过紧急补充氧气恢复亚硫酸盐水平的成像。在缺氧条件下活肺细胞中亚硫酸盐水平的成像能力，BFSF与本文的信息一起对于研究与麻醉相关的生物学指标具有意义。
