METHODS: Circulating CD4+ T cells from CD patients were analyzed by flow cytometry following the MBM program. Patients were randomly assigned to either a guided intervention group (IG) or a self-guided waitlist control group (CG) over a 9-month trial and compared with healthy blood donors.
RESULTS: Lifestyle intervention reduced regulatory T cell (Treg) frequencies in the blood of CD patients. Notably, we observed a significant correlation between the quality of life improvement and Treg frequencies in the IG but not in the CG. Furthermore, differential activation and expression of the gut-homing molecules G protein-coupled receptor 15 and CCR9 on circulating Tregs and CD4+ effector T cells were detected in both the IG and CG.
CONCLUSIONS: The MBM program, whether guided or self-directed, has the potential to restore the CD4+ T cell profile of CD patients to levels comparable to healthy blood donors. Lifestyle interventions may benefit CD progression, symptoms, and immunological status, but further analysis is needed to substantiate these findings and to fully understand their clinical implications. (ClinicalTrials.gov: NCT05182645).
Stress significantly impacts Crohn’s disease. Lifestyle intervention reduces circulating regulatory T cell frequencies, correlates with improved patient quality of life, holds promise for restoring circulating CD4+ T cell profiles, and improves patient care by integrating stress management.
方法:根据MBM程序通过流式细胞术分析来自CD患者的循环CD4+T细胞。在9个月的试验中,患者被随机分配到指导干预组(IG)或自我指导候补对照组(CG),并与健康献血者进行比较。
结果:生活方式干预降低了CD患者血液中调节性T细胞(Treg)的频率。值得注意的是,我们观察到生活质量改善与IG中的Treg频率之间存在显着相关性,但与CG中的Treg频率无关。此外,在IG和CG中均检测到循环Tregs和CD4效应T细胞上肠归巢分子G蛋白偶联受体15和CCR9的差异激活和表达。
结论:MBM计划,无论是引导还是自我引导,有可能将CD患者的CD4+T细胞谱恢复到与健康献血者相当的水平。生活方式干预可能有益于CD进展,症状,和免疫状态,但需要进一步的分析来证实这些发现并充分理解其临床意义.(ClinicalTrials.gov:NCT05182645)。
压力显著影响克罗恩病。生活方式干预降低循环调节性T细胞频率,与改善患者生活质量相关,有望恢复循环CD4+T细胞谱,并通过整合压力管理来改善患者护理。