Abstract:
High temperature (HT) severely restricts plant growth, development, and productivity. Plants have evolved a set of mechanisms to cope with HT, including the regulation of heat stress transcription factors (Hsfs) and heat shock proteins (Hsps). However, it is not clear how the transcriptional and translational levels of Hsfs and Hsps are controlled in tomato. Here, we reported that the HT-induced transcription factor SlWRKY55 recruited SlVQ11 to coordinately regulate defense against HT. SlWRKY55 directly bound to the promoter of SlHsfA2 and promoted its expression, which was increased by SlVQ11. Moreover, both SlWRKY55 and SlVQ11 physically interacted with SlHsfA2 to enhance the transcriptional activity of SlHsfA2. Thus, our results revealed a molecular mechanism that the SlWRKY55/SlVQ11-SlHsfA2 cascade enhanced thermotolerance and provided potential target genes for improving the adaptability of crops to HT.
摘要:
高温严重制约着植物的生长,发展,和生产力。植物已经进化出一套机制来应对HT,包括热应激转录因子(Hsfs)和热休克蛋白(Hsps)的调控。然而,目前尚不清楚如何控制番茄中Hsfs和Hsps的转录和翻译水平。这里,我们报道了HT诱导的转录因子SlWRKY55招募SlVQ11来协调调节针对HT的防御。SlWRKY55直接与SlHsfA2的启动子结合并促进其表达,由SlVQ11增加。此外,SlWRKY55和SlVQ11均与SlHsfA2物理相互作用以增强SlHsfA2的转录活性。因此,我们的结果揭示了SlWRKY55/SlVQ11-SlHsfA2级联增强耐热性的分子机制,并为改善作物对HT的适应性提供了潜在的靶基因。