PDF(Pubmed)
Abstract：
UNASSIGNED: Bovine viral diarrhea (BVD) disease is a viral infection in cows caused by a single-stranded plus-sense RNA virus of the Pestivirus genus under the Flaviviridae family. The clinical manifestation of BVD mainly includes diarrhea and immunosuppression, thereby exacerbating various respiratory diseases. This study was conducted to detect and molecularly characterize the bovine viral diarrhea disease virus (BVDV) in cattle on selected farms in Selangor, Malaysia.
UNASSIGNED: A reverse transcription polymerase chain reaction (RT-PCR) was performed for antigen detection in 253 plasma samples collected from cows using a cross-sectional study design. We selected the 5 untranslated regions (5\'-UTR) region and the E2 region to compare the genetic differences between the isolates.
UNASSIGNED: One sample was found to be positive (1/253) following RT-PCR targeting the conserved 5\'-UTR region of BVDV. Thus, BVDV antigen prevalence was 0.40% (95% confidence interval: 0.0%-2.2%). By targeting the hypervariable E2 region of the isolated virus, UPM/MAL/BVDV/D17, the virus was classified under the subgenotype BVDV-1a.
UNASSIGNED: BVDV is present and circulating on selected cattle farms in Selangor, Malaysia. Given the presence of BVDV in several subgenotypes, the screening of all incoming cattle at Malaysia\'s border is pertinent to prevent the entry of other BVDV subgenotypes into the country.
UNASSIGNED: A reverse transcription polymerase chain reaction (RT-PCR) was performed for antigen detection in 253 plasma samples collected from cows using a cross-sectional study design. We selected the 5 untranslated regions (5\'-UTR) region and the E2 region to compare the genetic differences between the isolates.
UNASSIGNED: One sample was found to be positive (1/253) following RT-PCR targeting the conserved 5\'-UTR region of BVDV. Thus, BVDV antigen prevalence was 0.40% (95% confidence interval: 0.0%-2.2%). By targeting the hypervariable E2 region of the isolated virus, UPM/MAL/BVDV/D17, the virus was classified under the subgenotype BVDV-1a.
UNASSIGNED: BVDV is present and circulating on selected cattle farms in Selangor, Malaysia. Given the presence of BVDV in several subgenotypes, the screening of all incoming cattle at Malaysia\'s border is pertinent to prevent the entry of other BVDV subgenotypes into the country.
摘要：
牛病毒性腹泻（BVD）病是由黄病毒科的瘟病毒属的单链正义RNA病毒引起的牛的病毒感染。BVD的临床表现主要为腹泻和免疫抑制,从而加剧各种呼吸道疾病。这项研究是为了检测和分子特征的牛病毒性腹泻病病毒（BVDV）在雪兰冶市选定的农场，马来西亚。
■进行逆转录聚合酶链反应（RT-PCR），用于使用横断面研究设计从奶牛收集的253个血浆样品中的抗原检测。我们选择了5个非翻译区（5'-UTR）区和E2区来比较分离株之间的遗传差异。
■在靶向BVDV的保守5'-UTR区的RT-PCR后发现一个样品是阳性的(1/253)。因此，BVDV抗原患病率为0.40％（95％置信区间：0.0％-2.2％）。通过靶向分离病毒的高变区E2，UPM/MAL/BVDV/D17，该病毒分为BVDV-1a亚型。
■BVDV存在并在Selangor的选定养牛场中流通，马来西亚。鉴于BVDV在几种亚型中的存在，在马来西亚边境对所有传入的牛进行筛查，以防止其他BVDV亚型进入该国。
■进行逆转录聚合酶链反应（RT-PCR），用于使用横断面研究设计从奶牛收集的253个血浆样品中的抗原检测。我们选择了5个非翻译区（5'-UTR）区和E2区来比较分离株之间的遗传差异。
■在靶向BVDV的保守5'-UTR区的RT-PCR后发现一个样品是阳性的(1/253)。因此，BVDV抗原患病率为0.40％（95％置信区间：0.0％-2.2％）。通过靶向分离病毒的高变区E2，UPM/MAL/BVDV/D17，该病毒分为BVDV-1a亚型。
■BVDV存在并在Selangor的选定养牛场中流通，马来西亚。鉴于BVDV在几种亚型中的存在，在马来西亚边境对所有传入的牛进行筛查，以防止其他BVDV亚型进入该国。
