PDF(Pubmed)
Abstract：
Since the use of antibiotics as growth promoters in animal feed has been restricted or banned in several countries, finding suitable alternatives is crucial for maintaining animal health. In this study, a novel formate acidifier named sodium diformate (NaDF) was synthesized, and the effects on growth performance and the prevention effects against Salmonella enterica serovar Pullorum infections in chickens were assessed. In broilers, NaDF supplementation improved growth performance, as evidenced by increased body weights and reduced feed conversion ratios. At 38 days of age, NaDF supplementation increased the levels of growth-hormone and ghrelin in the serum, lowered pH values in the gut, improved duodenal morphology, as shown by increased villus length/crypt depth ratios. NaDF also modulated the abundance of beneficial and harmful bacteria without changing the general microbiota diversity and short-chain fatty acids levels, which would be beneficial for maintaining gut homeostasis during its use. NaDF exhibited a broad spectrum of antibacterial activity in vitro. Supplementation with NaDF effectively decreased S. Pullorum colonization in the cecum, liver and spleen in chickens, and mitigated pathological changes in the tissues. Therefore, as a novel acidifier, NaDF can improve chicken growth performance by increasing growth-related hormones levels while maintaining the diversity of gut microbiota, and also resist intestinal bacterial infection. These results provided evidences for the application of NaDF as an effective and safe animal feed in poultry farming.
摘要：
由于在一些国家限制或禁止在动物饲料中使用抗生素作为生长促进剂，寻找合适的替代品对于维持动物健康至关重要。在这项研究中,合成了一种新型甲酸酸化剂二甲酸钠(NaDF),并评估了对鸡生长性能的影响和对肠沙门氏菌感染的预防作用。在肉鸡中，补充NaDF提高了生长性能，体重增加和饲料转化率降低证明了这一点。在38天大的时候，补充NaDF会增加血清中生长激素和生长素释放肽的水平，降低肠道的pH值，改善十二指肠形态，如增加的绒毛长度/隐窝深度比所示。NaDF还调节了有益和有害细菌的丰度，而不会改变一般微生物群的多样性和短链脂肪酸水平。这将有利于在使用过程中保持肠道稳态。NaDF在体外表现出广谱抗菌活性。补充NaDF可有效减少盲肠中的鸡白肠球菌定植，鸡的肝脏和脾脏，并减轻组织的病理变化。因此,作为一种新型酸化剂，NaDF可以通过增加生长相关激素水平来改善鸡的生长性能，同时保持肠道菌群的多样性，还能抵抗肠道细菌感染。这些结果为NaDF作为一种有效和安全的动物饲料在家禽养殖中的应用提供了依据。
