UNASSIGNED: Histopathological changes and inflammatory cytokine levels in a deep venous thrombosis (DVT) mouse model were assessed using HE staining and ELISA assays. A luciferase reporter assay was employed to identify the miR-21-3p and RRAGB targeting relationship. EPC proliferation, migration, and tube formation were evaluated through CCK-8, Transwell, and tubule formation assays, while the mTOR pathway and autophagy-related proteins were analyzed by immunofluorescence staining and western blotting.
UNASSIGNED: Berberine significantly improved EPC functions, such as proliferation, migration, and tube formation in vitro, and enhanced in vivo EPC-mediated wound healing in a DVT mouse model. Furthermore, miR-21-3p was downregulated in EPCs from VLU patients, and its overexpression improved model EPC functions. Mechanistically, RRAGB, which regulates the mTOR pathway, was identified as a potential miR-21-3p target in EPCs. Overexpression of RRAGB inhibited autophagic activity and impaired EPC function.
UNASSIGNED: Berberine shows promise in ameliorating EPC function and promoting wound healing in VLUs. The regulation of the miR-21-3p/RRAGB axis by berberine could offer a promising therapeutic approach for managing VLUs.
使用HE染色和ELISA测定评估深静脉血栓形成(DVT)小鼠模型中的组织病理学变化和炎性细胞因子水平。采用荧光素酶报告基因测定来鉴定miR-21-3p和RRAGB靶向关系。EPC增殖,迁移,通过CCK-8、Transwell、和小管形成测定,而mTOR通路和自噬相关蛋白通过免疫荧光染色和免疫印迹分析。
■小檗碱显著改善了EPC功能,如扩散,迁移,和体外试管形成,并在DVT小鼠模型中增强体内EPC介导的伤口愈合。此外,miR-21-3p在VLU患者的EPCs中下调,及其过表达改进了模型EPC功能。机械上,RRAGB,调节mTOR通路,被鉴定为EPCs中潜在的miR-21-3p靶标。RRAGB过表达抑制自噬活性和EPC功能受损。
■小檗碱在改善EPC功能和促进VLU伤口愈合方面显示出希望。小檗碱对miR-21-3p/RRAGB轴的调节可以为管理VLU提供有希望的治疗方法。