PDF(Pubmed)
Abstract：
Studies in fruit flies, Drosophila melanogaster, have observed considerable variation in the effect of dietary protein restriction (PR) on various fitness traits. In addition, not only are there inconsistent results relating lifespan to stress resistance, but also the long-term effects of PR are unexplored. We study PR implementation across generations (long term) hypothesizing that it will be beneficial for fitness traits, stress resistance and storage reserves due to nutritional plasticity transferred by parents to offspring in earlier Drosophila studies. By imposing two concentrations of PR diets (50% and 70% of control protein) from the pre-adult and adult (age 1 day) stages of the flies, we assessed the stage-specific and long-term effect of the imposed PR. All long-term PR flies showed increased resistance against the tested stressors (starvation, desiccation, H2O2-induced oxidative stress). In addition, we also found long-term PR-induced increased stress resistance across generations. The PR flies also possessed higher protein and triglyceride (TG) content, reduced glucose and unaffected glycogen levels. We also assayed the effect of returning the PR flies to control (AL) food for a single generation and assessed their biochemical parameters to witness the transient PR effect. It was seen that TG content upon reversal was similar to AL flies except for PRI70 males; however, the glucose levels of PR males increased, while they were consistently lower in females. Taken altogether, our study suggests that long-term PR implementation contributes to increased stress resistance and was found to influence storage reserves in D. melanogaster.
摘要：
对果蝇的研究，黑腹果蝇,已经观察到饮食蛋白限制（PR）对各种适应性特征的影响存在很大差异。此外,不仅寿命与抗应激性相关的结果不一致，但公关的长期影响也未被探索。我们研究了跨代（长期）的PR实施，假设它对健身特征有益，在早期的果蝇研究中，由于父母将营养可塑性转移给后代而产生的抗逆性和储存储备。通过施加两种浓度的PR饮食（50％和70％的对照蛋白质）从成年前和成年（年龄1天）阶段的苍蝇，我们评估了施加PR的阶段性和长期效应.所有长期PR果蝇对测试的应激源（饥饿，干燥,H2O2诱导的氧化应激)。此外,我们还发现长期PR诱导的跨代应激抗性增加。PR果蝇还具有较高的蛋白质和甘油三酯（TG）含量，降低葡萄糖和不受影响的糖原水平。我们还分析了将PR果蝇返回到控制（AL）食物中一代的效果，并评估了它们的生化参数以见证瞬时PR效应。可以看出，除PRI70雄性外，逆转后的TG含量与AL蝇相似；但是，男性PR的血糖水平升高,而女性则一直较低。总之，我们的研究表明,长期实施PR有助于提高抗逆性,并影响黑腹D.
