PDF(Pubmed)
Abstract：
BACKGROUND: Incontinence-associated dermatitis (IAD) is a tough problem in clinical settings, not only increasing the risk of complications like catheter-related urinary tract infections and pressure ulcers in elderly and critically ill patients, but also prolonging hospital stays, raising hospital costs, and possibly leading to medical disputes. This study is aimed to evaluate the therapeutic effect of silicone dressing combined with topical oxygen therapy on IAD in a rat model.
METHODS: An IAD rat model induced by synthetic urine with trypsin was established. Hematoxylin & eosin staining was carried out to examine skin histology. Using immunofluorescence, the microvessel density in the affected skin tissues was determined. ELISA was performed to measure the concentrations of inflammatory cytokines and angiogenic factors in serum. The mRNA expression of EGF, PDGF, and VEGF was detected via qRT-PCR. Western blotting was employed to determine NF-κB p65/STAT1 pathway-related protein levels.
RESULTS: Compared to single therapy, silicone dressing combined with topical oxygen therapy could significantly reduce the severity of IAD, improve skin histology, inhibit inflammation, and promote angiogenesis in IAD rat models. Additionally, the results showed that relatively speaking, the combined therapy suppressed the NF-κB p65/STAT1 signaling pathway more effectively.
CONCLUSIONS: These findings indicated that silicone dressing combined with topical oxygen therapy can alleviate IAD through promoting wound healing and inhibiting inflammation via NF-κB p65/STAT1 signaling pathway in a rat model, which provided a theoretical basis for the prevention and treatment of IAD in clinic.
METHODS: An IAD rat model induced by synthetic urine with trypsin was established. Hematoxylin & eosin staining was carried out to examine skin histology. Using immunofluorescence, the microvessel density in the affected skin tissues was determined. ELISA was performed to measure the concentrations of inflammatory cytokines and angiogenic factors in serum. The mRNA expression of EGF, PDGF, and VEGF was detected via qRT-PCR. Western blotting was employed to determine NF-κB p65/STAT1 pathway-related protein levels.
RESULTS: Compared to single therapy, silicone dressing combined with topical oxygen therapy could significantly reduce the severity of IAD, improve skin histology, inhibit inflammation, and promote angiogenesis in IAD rat models. Additionally, the results showed that relatively speaking, the combined therapy suppressed the NF-κB p65/STAT1 signaling pathway more effectively.
CONCLUSIONS: These findings indicated that silicone dressing combined with topical oxygen therapy can alleviate IAD through promoting wound healing and inhibiting inflammation via NF-κB p65/STAT1 signaling pathway in a rat model, which provided a theoretical basis for the prevention and treatment of IAD in clinic.
摘要：
背景：失禁相关性皮炎（IAD）在临床上是一个棘手的问题，不仅增加了老年和危重患者发生导尿管相关尿路感染和压疮等并发症的风险，而且延长了住院时间，提高医院费用，可能导致医疗纠纷。本研究旨在评价硅胶敷料联合局部氧疗对大鼠IAD的治疗效果。
方法：建立胰蛋白酶合成尿液诱导的IAD大鼠模型。进行苏木精&伊红染色以检查皮肤组织学。使用免疫荧光，确定受影响的皮肤组织中的微血管密度。进行ELISA以测量血清中炎性细胞因子和血管生成因子的浓度。EGF的mRNA表达,PDGF,通过qRT-PCR检测VEGF。采用蛋白质印迹法测定NF-κBp65/STAT1通路相关蛋白水平。
结果：与单一疗法相比，硅胶敷料联合局部氧疗可显着降低IAD的严重程度，改善皮肤组织学，抑制炎症，并促进IAD大鼠模型的血管生成。此外,结果表明，相对而言，联合治疗能更有效地抑制NF-κBp65/STAT1信号通路。
结论：这些结果表明，硅胶敷料联合局部氧疗可通过NF-κBp65/STAT1信号通路促进创面愈合和抑制炎症，从而减轻大鼠模型的IAD。为IAD的临床防治提供了理论依据。
方法：建立胰蛋白酶合成尿液诱导的IAD大鼠模型。进行苏木精&伊红染色以检查皮肤组织学。使用免疫荧光，确定受影响的皮肤组织中的微血管密度。进行ELISA以测量血清中炎性细胞因子和血管生成因子的浓度。EGF的mRNA表达,PDGF,通过qRT-PCR检测VEGF。采用蛋白质印迹法测定NF-κBp65/STAT1通路相关蛋白水平。
结果：与单一疗法相比，硅胶敷料联合局部氧疗可显着降低IAD的严重程度，改善皮肤组织学，抑制炎症，并促进IAD大鼠模型的血管生成。此外,结果表明，相对而言，联合治疗能更有效地抑制NF-κBp65/STAT1信号通路。
结论：这些结果表明，硅胶敷料联合局部氧疗可通过NF-κBp65/STAT1信号通路促进创面愈合和抑制炎症，从而减轻大鼠模型的IAD。为IAD的临床防治提供了理论依据。
