Abstract：
The utilization of nitenpyram for aphid and whitefly control may induce environmental contamination and negative repercussions on non-target organisms. Formerly, we found that nitenpyram would pollute the peripheral and sub-peripheral areas of the adjacent mulberry orchard. Under acute toxicity conditions, nitenpyram induced oxidative damage in silkworms, affected biological metabolism, synthesis, immunity, and signal transduction. Considering the impact of nitenpyram mist drift on mulberry leaves, we investigated the effects of low concentrations of nitenpyram on silkworms. The results showed that silkworms exposed to 0.17 mg/L, 0.35 mg/L and 0.70 mg/L of nitenpyram (1/40 LC50, 1/20 LC50 and 1/10 LC50) showed obvious poisoning symptoms. The cocoon weight and cocoon shell weight decreased gradually with increases in the concentration, and these decreases prolonged the growth and development time of silkworms and induced the detoxification enzymes carboxylesterase (CarE) and glutathione-S-transferase (GST) to cope with the stress damage caused by nitenpyram. Exposure to low concentrations of nitenpyram downregulates genes involved in the drug metabolism-other enzymes and peroxisome pathway in silkworms. Additionally, through injection of miRNA mimics and inhibitors, we discovered that detoxifying enzyme pathway genes are influenced by bmo-miR-3382-3P, bmo-miR-3213-5P and bmo-miR-133, regulating the immune response of silkworms. This study provides an overall view of the toxicity and detoxification metabolism of nitenpyram in silkworm, and provides a reference for environmental assessment.
摘要：
利用烯丙啶防治蚜虫和粉虱可能会引起环境污染和对非目标生物的负面影响。以前，我们发现亚硝胺会污染邻近桑园的外围和外围区域。在急性毒性条件下，氮丙啶对家蚕的氧化损伤，影响生物代谢，合成，豁免权,和信号转导。考虑到氮丙啶雾漂移对桑叶的影响，我们研究了低浓度的氮吡喃对家蚕的影响。结果表明,家蚕暴露于0.17mg/L,0.35mg/L和0.70mg/L的硝啶（1/40LC50，1/20LC50和1/10LC50）均表现出明显的中毒症状。随着浓度的增加，茧重量和茧壳重量逐渐降低，这些减少延长了家蚕的生长发育时间，并诱导了解毒酶羧酸酯酶（CarE）和谷胱甘肽-S-转移酶（GST）以应对烯丙胺引起的胁迫损伤。暴露于低浓度的氮吡喃下调了参与药物代谢的基因-家蚕的其他酶和过氧化物酶体途径。此外,通过注射miRNA模拟物和抑制剂，我们发现解毒酶途径基因受bmo-miR-3382-3P的影响，bmo-miR-3213-5P和bmo-miR-133调控家蚕的免疫应答。本研究从总体上对家蚕烯丙啶的毒性和解毒代谢,为环境评价提供参考。
