Abstract：
Nanoplastics at environmentally relevant concentrations (ERCs) could cause transgenerational toxicity on organisms. Caenorhabditis elegans is an important model for the study of transgenerational toxicology of pollutants. Nevertheless, the underlying mechanisms for the control of transgenerational nanoplastic toxicity by germline signals remain largely unclear. In C. elegans, exposure to 1-100 μg/L polystyrene nanoparticle (PS-NP) decreased expression of germline ced-1 encoding a G protein-coupled receptor at parental generation (P0-G). After PS-NP exposure at P0-G, transgenerational decrease in germline ced-1 expression could be detected. Meanwhile, the susceptibility to transgenerational PS-NP toxicity was observed in ced-1(RNAi) animals. After PS-NP exposure at P0-G, germline RNAi of ced-1 increased expressions of met-2 and set-6 encoding histone methylation transferases. The susceptibility of ced-1(RNAi) to transgenerational PS-NP toxicity could be inhibited by RNAi of met-2 and set-6. Moreover, in PS-NP exposed met-2(RNAi) and set-6(RNAi) nematodes, expressions of ins-39, wrt-3, and/or efn-3 encoding secreted ligands were decreased. Therefore, our results demonstrated that inhibition in germline CED-1 mediated the toxicity induction of nanoplastics at ERCs across multiple generations in nematodes.
摘要：
环境相关浓度的纳米塑料（ERC）可能会对生物体产生跨代毒性。秀丽隐杆线虫是研究污染物跨代毒理学的重要模型。然而，通过种系信号控制跨代纳米塑料毒性的潜在机制仍不清楚.在秀丽隐杆线虫中，暴露于1-100μg/L聚苯乙烯纳米颗粒（PS-NP）降低了亲本世代编码G蛋白偶联受体的种系ced-1的表达（P0-G）。PS-NP暴露于P0-G后，可以检测到种系ced-1表达的代际减少。同时,在ced-1（RNAi）动物中观察到对跨代PS-NP毒性的易感性。PS-NP暴露于P0-G后，ced-1的种系RNAi增加了编码组蛋白甲基化转移酶的met-2和set-6的表达。met-2和set-6的RNAi可以抑制ced-1（RNAi）对跨代PS-NP毒性的敏感性。此外，在PS-NP暴露的met-2（RNAi）和set-6（RNAi）线虫中，编码分泌配体的ins-39，wrt-3和/或efn-3的表达降低。因此,我们的结果表明，在线虫中，种系CED-1的抑制作用介导了纳米塑料在ERC上的毒性诱导。
