Abstract：
Well plates are widely used in biological experiments, particularly in pharmaceutical sciences and cell biology. Its popularity stems from its versatility to support a variety of fluorescent markers for high throughput monitoring of cellular activities. However, using fluorescent markers in traditional well plates has its own challenges, namely, they can be potentially toxic to cells, and thus, may perturb their biological functions; and it is difficult to monitor multiple analytes concurrently and in real-time inside each well. This paper presents a fully instrumented microphysiological system with integrated sensors (IMSIS) with a similar well format. Each well in the microphysiological system has a set of sensors for monitoring multiple metabolic analytes in real-time. The IMSIS platform is supported by integrated bioelectronic circuits and a graphical user interface for easy user configuration and monitoring. The system has integrated microfluidics to maintain its microphysiological environment within each well. The IMSIS platform currently incorporates O2, H2O2, and pH sensors inside each well, allowing up to six wells to perform concurrent measurements in real-time. Furthermore, the architecture is scalable to achieve an even higher level of throughput. The miniaturized design ensures portability, suitable for small offices and field applications. The IMSIS platform was successfully used to monitor in real-time the mitochondrial functions of live bovine embryos in O2 consumption, H2O2 release as an indication of ROS production, and extracellular acidity changes before and after the introduction of external substrates.
摘要：
孔板广泛应用于生物实验,特别是在药物科学和细胞生物学。其受欢迎程度源于其多功能性,以支持用于细胞活性的高通量监测的多种荧光标记。然而,在传统孔板中使用荧光标记有其自身的挑战，即,它们可能对细胞有潜在的毒性，因此，可能扰乱它们的生物学功能;并且难以在每个井内同时和实时地监测多个分析物。本文介绍了一种具有类似井格式的集成传感器（IMSIS）的完全仪器化的微生理系统。微生理系统中的每个孔具有用于实时监测多种代谢分析物的一组传感器。IMSIS平台由集成生物电子电路和图形用户界面支持，便于用户配置和监控。该系统具有集成的微流体以在每个井内维持其微生理环境。IMSIS平台目前在每个井内集成了O2，H2O2和pH传感器，允许多达六口井进行实时并行测量。此外，该架构是可扩展的，以实现更高的吞吐量水平。小型化设计确保便携性，适用于小型办公室和现场应用。IMSIS平台成功用于实时监测氧气消耗中活牛胚胎的线粒体功能，H2O2释放作为ROS产生的指标，和外部底物引入前后的细胞外酸度变化。
