Abstract:
Using a chemiluminescence reaction between luminol and H2O2 in basic solution, an ultrasensitive electrochemiluminescence (ECL) aptasensor was developed for the determination of tobramycin (TOB), as an aminoglycoside antibiotic. Ti3C2/Ni/Sm-LDH-based nanocomposite effectively catalyzes the oxidation of luminol and decomposition of H2O2, leading to the formation of different reactive oxygen species (ROSs), thus amplifying the ECL signal intensity of luminol, which can be used for the determination of TOB concentration. To evaluate the performance of the electrochemiluminescence aptasensor and synthesized nanocomposite, different methods such as cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) analyses were performed. The considerable specific area, large number of active sites, and enhanced electron transfer reaction on this nanocomposite led to the development of an ECL aptasensor with high sensitivity and electrocatalytic activity. After optimizing the preparation method and analysis conditions, the aptasensor revealed a wide linear response ranging from 1.0 pM to 1.0 μM with a detection limit of 18 pM, displaying outstanding accuracy, specificity, and response stability. The developed ECL sensor was found to be applicable to the determination of TOB in human serum samples and is anticipated to possess excellent clinical potentials for detecting other antibiotics, as well.
摘要:
利用鲁米诺和H2O2在碱性溶液中的化学发光反应,开发了一种超灵敏的电化学发光(ECL)aptasensor,用于测定妥布霉素(TOB),作为氨基糖苷类抗生素.Ti3C2/Ni/Sm-LDH基纳米复合材料有效催化鲁米诺的氧化和H2O2的分解,导致形成不同的活性氧(ROSs),从而放大鲁米诺的ECL信号强度,可用于TOB浓度的测定。为了评估电化学发光传感器和合成的纳米复合材料的性能,进行了不同的方法,例如循环伏安法(CV)和电化学阻抗谱(EIS)分析。相当大的特定区域,大量活跃的网站,在这种纳米复合材料上增强的电子转移反应导致了具有高灵敏度和电催化活性的ECLaptasensor的开发。在优化制备方法和分析条件后,aptasensor揭示了从1.0pM到1.0μM的宽线性响应,检测限为18pM,显示出众的准确性,特异性,和响应稳定性。已开发的ECL传感器适用于测定人血清样品中的TOB,并有望具有检测其他抗生素的出色临床潜力。也是。