PDF(Pubmed)
Abstract：
This study aims to investigate the effect of Mineral Trioxide Aggregate (MTA), a bioactive endodontic cement, and Concentrated Growth Factor (CGF), a second-generation autologous growth factor, on pulpotomy-induced pulp inflammation. The study utilized the maxillary anterior central teeth of thirty-six young male Sprague Dawley rats. Forty-eight teeth were randomly assigned to two groups (12 rats/group; 24 teeth/group) based on the capping material (MTA or CGF). Subsequently, two subgroups (MTAG and CGFG) were formed per group (12 teeth/group) based on the time following pulpotomy (2-weeks and 4-weeks). The central teeth of the 12 animals assigned to the control group (CG) were not manipulated in any way, both in the 2-week group and in the 4-week group. Tissue samples extracted from rats at the end of the experiment were stained with H&E for histopathological analysis. For immunohistochemical analysis, primary antibodies for TNF-α and NF-kβ/65 were incubated. Data obtained from semi-quantitative analysis were assessed for normal distribution using Skewness-Kurtosis values, Q-Q plot, Levene\'s test, and the Shapiro-Wilk test on statistical software. A P value < 0.05 was considered significant. When compared with the control group, both MTAG and CGFG showed increased edematous and inflammatory areas. In MTAG, edematous and inflammatory areas decreased significantly from the 2nd week (2(2-2), 2(1-2)) to the 4th week (1(1-1), 1(0-1)), while in CGFG, edematous areas decreased (2(2-3), 1.5(1-2)), and inflammatory areas increased significantly (2(2-3), 3(2-2.5)). When compared with the control group, TNF-α and NF-kβ/p65 positivity were higher in both MTAG and CGFG. In MTAG, TNF-α [2(1.5-2)] and NF-kβ/p65 [1.5(1-2)] positivity decreased significantly from the 2nd week to the 4th week [TNF-α: 1(1-1), NF-kβ/p65: 1(1-2)], while no significant change was observed in CGFG. In conclusion, this study revealed a reduction in cells showing TNF-α and NF-kβ/p65 positivity in the MTA treatment group compared to the CGF group. Although MTA demonstrated more favorable results than CGF in mitigating pulpal inflammation within the scope of this study, further experimental and clinical investigations are warranted to obtain comprehensive data regarding CGF.
摘要：
本研究旨在探讨矿物三氧化物聚集体(MTA)的作用,一种生物活性的牙髓水泥，和浓缩生长因子（CGF），第二代自体生长因子，关于牙髓切除术引起的牙髓炎症。该研究利用了36只年轻雄性SpragueDawley大鼠的上颌中央前牙。根据盖帽材料（MTA或CGF），将48颗牙齿随机分为两组（12只大鼠/组；24颗牙齿/组）。随后，根据牙髓切除术后的时间(2周和4周),每组(12颗牙齿/组)形成两个亚组(MTAG和CGFG).没有以任何方式操作分配给对照组（CG）的12只动物的中央牙齿，在2周组和4周组。在实验结束时从大鼠提取的组织样品用H&E染色用于组织病理学分析。对于免疫组织化学分析，孵育TNF-α和NF-kβ/65的一级抗体。从半定量分析获得的数据使用偏度-峰度值评估正态分布，Q-Q图,Levene\'stest,和统计软件上的Shapiro-Wilk检验。P值<0.05被认为是显著的。与对照组相比，MTAG和CGFG均显示水肿和炎症区域增加.在MTAG中,水肿和炎症区域从第二周开始显着减少（2（2-2），2（1-2））至第4周（1（1-1），1（0-1），而在CGFG，水肿面积减少（2（2-3），1.5(1-2)),和炎症区域显着增加（2（2-3），3（2-2.5））。与对照组相比，MTAG和CGPG中TNF-α和NF-kβ/p65阳性均较高。在MTAG中,TNF-α[2（1.5-2）]和NF-kβ/p65[1.5（1-2）]阳性从第2周到第4周显着降低[TNF-α：1（1-1），NF-kβ/p65:1(1-2)]，而CGFG没有观察到显著变化。总之,这项研究显示，与CGF组相比，MTA治疗组中显示TNF-α和NF-kβ/p65阳性的细胞减少。尽管在本研究范围内，MTA在减轻牙髓炎症方面比CGF表现出更有利的结果，需要进一步的实验和临床研究,以获得有关CGF的全面数据.
