PDF(Pubmed)
Abstract：
Canada thistle is a pervasive perennial weed, causing challenges to agricultural and natural ecosystems globally. Although research has focused on the phenology, genetics, and control of Canada thistle, little is known about the rhizosphere microbiome and the role plant-microbe interactions play in invasion success. This study investigated the rhizosphere microbiome of Canada thistle across diverse climates, soils, and crops in the U.S. northern Great Plains. Soil and rhizosphere samples were collected and bacterial 16S and fungal ITS2 sequencing were performed to characterize the core microbiome and identify potential factors contributing to invasion success. Amplicon sequencing revealed a stable core microbiome that was detected in the Canada thistle rhizosphere across all locations. The core microbiome was dominated by the bacterial phyla Actinobacteriota and Proteobacteria and fungal phyla Ascomycota and Basidiomycota. Differential abundance analysis showed rhizosphere fungal communities were enriched in pathogen-containing genera with a 1.7-fold greater abundance of Fusaria and a 2.6-fold greater abundance of Gibberella compared to bulk soil. Predictive functional profiling showed rhizosphere communities were enriched (p < 0.05, FDR corrected) in plant pathogen fungal guilds which represented 19% of the fungal community. The rhizosphere microbiome was similar in composition across environments, highlighting the stable association between Canada thistle and specific microbial taxa. This study characterized the core microbiome of Canada thistle, and the findings highlight plant-microbe interactions shaping invasive behavior. These findings are important for understanding the ecological impacts of plant invasion and soil-microbe ecological processes.
摘要：
加拿大蓟是一种普遍存在的多年生杂草，对全球农业和自然生态系统造成挑战。尽管研究集中在物候学上，遗传学,并控制加拿大蓟，对根际微生物组以及植物-微生物相互作用在入侵成功中的作用知之甚少。这项研究调查了加拿大蓟在不同气候下的根际微生物组，土壤,和美国北部大平原的农作物。收集土壤和根际样品，并进行细菌16S和真菌ITS2测序以表征核心微生物组并确定有助于入侵成功的潜在因素。Amplicon测序揭示了在所有地点的加拿大蓟根际中都检测到的稳定的核心微生物组。核心微生物组由细菌门放线菌和变形菌以及真菌门子囊菌和担子菌为主。差异丰度分析显示，根际真菌群落富含含病原体的属，与散装土壤相比，镰刀菌的丰度高1.7倍，赤霉素的丰度高2.6倍。预测功能分析显示，根际群落在植物病原体真菌行会中富集（p<0.05，FDR校正），占真菌群落的19％。不同环境的根际微生物组的组成相似，强调加拿大蓟和特定微生物类群之间的稳定关联。这项研究表征了加拿大蓟的核心微生物组，研究结果强调了植物-微生物相互作用塑造入侵行为。这些发现对于理解植物入侵和土壤微生物生态过程的生态影响非常重要。
