Abstract:
In order to investigate the changes in the properties of the cell culture solution in the effect of cell synchronization via cell starvation (for 12, 24, and 36 h), a new spiral-interdigital pattern of microelectrode as a biosensor has been proposed. Then, to test its superiority, the results of this spiral-interdigital pattern with the results of the commercial pattern have been compared. The cells were selected from breast cancer standard lines (MDA-MB-231). Changes in CV peaks of the secretions were recorded by the spiral-interdigital pattern, in which increasing the interactive surface with homogenous electric paths had been considered by simulation before fabrication. The results of the simulation and experimental procedures showed a meaningful correlation. The occurrence of CV oxidative peaks at about 0.1-0.4 V and reductive peaks at approximately 0 V in the spiral-interdigital biosensor in the starved MDA-MB-231 cell line has been observed. The starvation situation resembles one that does not cause meaningful cell apoptosis or necrosis, and this method is only used to make the cells synchronized. Also, no peak is observed in normal cell growth conditions. In addition, by using the commercial design of the electrodes, no peak is observed in any of the conditions of normal and synchronized growth of the cells. Therefore, it seems that the observed peaks are caused by the agents that are secreted in the cell culture solution in a synchronized situation. Moreover, the design of the new spiral-interdigital electrode can significantly increase the sensitivity of the sensor to receive these peaks due to more space and a uniform electric field.
摘要:
为了研究通过细胞饥饿(12、24和36h)对细胞同步化的影响中细胞培养液特性的变化,提出了一种新的螺旋叉指型微电极作为生物传感器。然后,为了测试它的优越性,将这种螺旋叉指模式的结果与商业模式的结果进行了比较。细胞选自乳腺癌标准品系(MDA-MB-231)。通过螺旋叉指模式记录分泌物的CV峰的变化,在制造之前,已经通过模拟考虑了增加具有均匀电路径的交互表面。仿真和实验程序的结果表明了有意义的相关性。在饥饿的MDA-MB-231细胞系中,在螺旋叉指型生物传感器中观察到约0.1-0.4V的CV氧化峰和约0V的还原峰。饥饿情况类似于不会引起有意义的细胞凋亡或坏死的情况,此方法仅用于使细胞同步。此外,在正常细胞生长条件下没有观察到峰值。此外,通过使用电极的商业设计,在细胞正常和同步生长的任何条件下都没有观察到峰值。因此,观察到的峰似乎是由在同步情况下分泌在细胞培养溶液中的试剂引起的。此外,由于更多的空间和均匀的电场,新型螺旋叉指电极的设计可以显着提高传感器接收这些峰值的灵敏度。