Abstract：
5-Hydroxytryptophan (5-HTP), a precursor of the neurotransmitter serotonin in mammals, has demonstrated efficacy in treating various diseases such as depression, fibromyalgia and obesity. However, conventional biosynthesis methods of 5-HTP are limited by low yield and high reagent and process costs. In this study, the strain C1T7-S337A/F318Y with optimized promoter distribution was obtained, and the 5-HTP yield was 60.30 % higher than that of the initial strain. An efficient fermentation process for 5-HTP synthesis was developed using strain C1T7-S337A/F318Y with whey powder as a substrate for cell growth and inducer production. Shake flask fermentation experiments yielded 1.302 g/L 5-HTP from 2.0 g/L L-tryptophan (L-Trp), surpassing the whole-cell biocatalysis by 42.86 %. Scale-up to a 5 L fermenter further increased the yield to 1.649 g/L. This fermentation strategy substantially slashed reagent cost by 95.39 %, providing a more economically viable and environmentally sustainable route for industrial biosynthesis of 5-HTP. Moreover, it contributes to the broader utilization of whey powder in various industries.
摘要：
5-羟色氨酸（5-HTP），哺乳动物神经递质血清素的前体，在治疗各种疾病如抑郁症方面表现出疗效，纤维肌痛和肥胖。然而,5-HTP的常规生物合成方法受低收率和高试剂和工艺成本的限制。在这项研究中,获得启动子分布优化的C1T7-S337A/F318Y菌株,5-HTP产率比初始菌株高60.30％。使用菌株C1T7-S337A/F318Y，以乳清粉为底物，开发了一种用于5-HTP合成的有效发酵工艺，用于细胞生长和诱导物的生产。摇瓶发酵实验从2.0g/LL-色氨酸（L-Trp）中获得1.302g/L5-HTP，超过全细胞生物催化42.86%。放大到5L发酵罐，产量进一步提高到1.649g/L。这种发酵策略大幅削减了95.39%的试剂成本，为5-HTP的工业生物合成提供更经济可行和环境可持续的路线。此外，它有助于乳清粉在各种行业中的广泛利用。
