METHODS: In this study, 30 patients with drug-resistant epilepsy (DRE), 30 patients with well-controlled epilepsy (WCE), and 29 healthy controls (HC) were enrolled. Multi-proinflammatory cytokines were measured by LUMINX multi-factor detection.
RESULTS: The levels of IL-1β, IL-7, IL-12, and IL-17 were significantly elevated, and the levels of CX3CL1 and ITAC were significantly decreased in epilepsy patients compared with healthy controls. Furthermore, the level of IL-17 was significantly higher in the DRE group compared to WCE. We also found the ratio of IL-7/CX3CL discriminates accurately between patients and controls, with a ROC Area Under the Curve (AUC) of 0.963 (P<0.001). The levels of IL-1β, IL-7, IL-12, and IL-17 in the DRE group were positively correlated with the National Hospital Seizure Severity Scale (NHS3) scores (IL-1β, P = 0.029; IL-12, P = 0.039; IL-17, P = 0.004). IL-17 was positively correlated with seizure frequency (P = 0.050), while ITAC was negatively correlated with seizure frequency (P = 0.012) and Sudden Unexpected Death in Epilepsy-3 (SUDEP-3) scores (P = 0.023).
CONCLUSIONS: IL-1β, IL-12, and IL-17 may be used to predict seizure severity and the IL-7/CX3CL1 ratio may be a candidate biomarker for predicting epileptic seizures. While CX3CL1 and ITAC play anti-epileptic effects, ITAC may be used to assess the risk of SUDEP.
方法:在本研究中,30例耐药癫痫(DRE),30例控制良好的癫痫患者(WCE),29名健康对照(HC)入组。通过LUMINX多因素检测检测多种促炎细胞因子。
结果:IL-1β水平,IL-7、IL-12和IL-17显著升高,与健康对照组相比,癫痫患者的CX3CL1和ITAC水平显着降低。此外,DRE组IL-17水平明显高于WCE.我们还发现IL-7/CX3CL的比例能准确区分患者和对照组,曲线下的ROC面积(AUC)为0.963(P<0.001)。IL-1β水平,DRE组IL-7、IL-12、IL-17与国家医院癫痫严重程度量表(NHS3)评分呈正相关(IL-1β,P=0.029;IL-12,P=0.039;IL-17,P=0.004)。IL-17与癫痫发作频率呈正相关(P=0.050),而ITAC与癫痫发作频率(P=0.012)和癫痫猝死-3(SUDEP-3)评分(P=0.023)呈负相关。
结论:IL-1β,IL-12和IL-17可用于预测癫痫发作严重程度,IL-7/CX3CL1比率可能是预测癫痫发作的候选生物标志物。虽然CX3CL1和ITAC具有抗癫痫作用,ITAC可用于评估SUDEP的风险。