Abstract：
Epstein-Barr virus (EBV) is the first human oncogenic virus known to express microRNAs (miRNAs), which are closely associated with the development of various tumors, including nasopharyngeal and gastric cancers. Stearoyl-CoA Desaturase 1 (SCD1) is a key enzyme in fatty acid synthesis, highly expressed in numerous tumors, promoting tumor growth and metastasis, making it a potential therapeutic target. In this study, we found that SCD1 expression in EBV-associated gastric cancer (EBVaGC) was significantly lower than in EBV-negative gastric cancer (EBVnGC) at both cellular and tissue levels. In addition, EBV-miR-BART20-5p targets the 3\'-UTR of SCD1, downregulating its expression. Moreover, overexpression of SCD1 in EBVaGC cells promoted cell migration and proliferation while inhibiting autophagy. These results suggest that EBV-encoded miRNA-BART20-5p may contribute to EBVaGC progression by targeting SCD1.
摘要：
EB病毒（EBV）是已知的第一个表达微小RNA（miRNA）的人类致癌病毒，与各种肿瘤的发展密切相关，包括鼻咽癌和胃癌.硬脂酰辅酶A去饱和酶1(SCD1)是脂肪酸合成的关键酶,在许多肿瘤中高表达，促进肿瘤生长和转移，使其成为潜在的治疗靶点。在这项研究中,我们发现,在细胞和组织水平上,EBV相关胃癌(EBVaGC)中的SCD1表达显著低于EBV阴性胃癌(EBVnGC).此外,EBV-miR-BART20-5p靶向SCD1的3'-UTR，下调其表达。此外，在EBVaGC细胞中过表达SCD1促进细胞迁移和增殖,同时抑制自噬。这些结果表明，EBV编码的miRNA-BART20-5p可能通过靶向SCD1促进EBVaGC进展。
