Abstract：
OBJECTIVE: Severe congenital neutropenia (SCN) is a raredisorder characterized by diminished neutrophil levels. Despite granulocytecolony-stimulating factor (G-CSF) treatment, SCN patients remain still prone tosevere infections, including periodontal disease-a significant oral healthrisk. This study investigates the host proteome and metaproteome in saliva andgingival crevicular fluid (GCF) of G-CSF-treated patients.
METHODS: We used label-free quantitative proteomics on saliva and GCF samples from SCN patients before (n = 10, mean age: 10.7 ± 6.6 years) and after a 6-month oral hygiene intervention (n = 9,mean age: 11.6 ± 5.27 years), and from 12 healthy controls.
RESULTS: We quantified 894 proteins in saliva (648 human,246 bacterial) and 756 proteins in GCF (493 human, 263 bacterial). Predominant bacterial genera included Streptococcus, Veillonella, Selenomonas, Corynebacterium, Porphyromonas, and Prevotella. SCN patients showed reduced antimicrobial peptides (AMPs) and elevated complement proteins compared tohealthy controls. Oral hygiene intervention improved oral epithelial conditionsand reduced both AMPs and complement proteins.
CONCLUSIONS: SCN patients have aunique proteomic profile with reduced AMPs and increased complement proteins, contributing to infection susceptibility. Oral hygiene intervention not onlyimproved oral health in SCN patients but also offers potential overall therapeuticbenefits.
METHODS: We used label-free quantitative proteomics on saliva and GCF samples from SCN patients before (n = 10, mean age: 10.7 ± 6.6 years) and after a 6-month oral hygiene intervention (n = 9,mean age: 11.6 ± 5.27 years), and from 12 healthy controls.
RESULTS: We quantified 894 proteins in saliva (648 human,246 bacterial) and 756 proteins in GCF (493 human, 263 bacterial). Predominant bacterial genera included Streptococcus, Veillonella, Selenomonas, Corynebacterium, Porphyromonas, and Prevotella. SCN patients showed reduced antimicrobial peptides (AMPs) and elevated complement proteins compared tohealthy controls. Oral hygiene intervention improved oral epithelial conditionsand reduced both AMPs and complement proteins.
CONCLUSIONS: SCN patients have aunique proteomic profile with reduced AMPs and increased complement proteins, contributing to infection susceptibility. Oral hygiene intervention not onlyimproved oral health in SCN patients but also offers potential overall therapeuticbenefits.
摘要：
目的：严重的先天性中性粒细胞减少症（SCN）是一种以中性粒细胞水平降低为特征的罕见疾病。尽管粒细胞集落刺激因子（G-CSF）治疗，SCN患者仍然容易发生严重感染，包括牙周病-一个重大的口腔健康风险。这项研究调查了G-CSF治疗患者的唾液和龈沟液（GCF）中的宿主蛋白质组和元蛋白质组。
方法：我们对SCN患者在口腔卫生干预前（n=10，平均年龄：10.7±6.6岁）和6个月后（n=9，平均年龄：11.6±5.27岁）的唾液和GCF样本进行了无标签定量蛋白质组学研究。和12个健康对照。
结果：我们定量了唾液中的894种蛋白质（648人，GCF中的246种细菌）和756种蛋白质（493人，263细菌）。主要细菌属包括链球菌，Veillonella,硒单胞菌,棒状杆菌,卟啉单胞菌,还有Prevotella.与健康对照组相比，SCN患者的抗菌肽（AMP）减少，补体蛋白升高。口腔卫生干预改善了口腔上皮状况，降低了AMPs和补体蛋白。
结论：SCN患者具有独特的蛋白质组学特征，AMPs减少，补体蛋白增加，有助于感染易感性。口腔卫生干预不仅改善了SCN患者的口腔健康，而且还提供了潜在的整体治疗益处。
方法：我们对SCN患者在口腔卫生干预前（n=10，平均年龄：10.7±6.6岁）和6个月后（n=9，平均年龄：11.6±5.27岁）的唾液和GCF样本进行了无标签定量蛋白质组学研究。和12个健康对照。
结果：我们定量了唾液中的894种蛋白质（648人，GCF中的246种细菌）和756种蛋白质（493人，263细菌）。主要细菌属包括链球菌，Veillonella,硒单胞菌,棒状杆菌,卟啉单胞菌,还有Prevotella.与健康对照组相比，SCN患者的抗菌肽（AMP）减少，补体蛋白升高。口腔卫生干预改善了口腔上皮状况，降低了AMPs和补体蛋白。
结论：SCN患者具有独特的蛋白质组学特征，AMPs减少，补体蛋白增加，有助于感染易感性。口腔卫生干预不仅改善了SCN患者的口腔健康，而且还提供了潜在的整体治疗益处。
