PDF(Pubmed)
Abstract：
CONCLUSIONS: Multilesional basal cell carcinoma (BCC) are spread on sun exposed skin areas, including arms, face and back. The first-line treatment remains the surgical resection or Mohs surgery. Despite its high complexity, Mohs surgery is well practiced in USA and Germany and presents very good results both in esthetic and in carcinology point of view. Large lesions more than 2 cm remain challenging to remove by topical cream used in photodynamic therapy (PDT). If these larger lesions are not treated in less than 1 month, they could grow deeply in the skin, thus enhancing the risk of reoccurrence and the severity of the disease. Despite this model herein studied, that is non melanoma skin cancer is a good prognostic cancer, the therapy aims to be applied to more aggressive melanoma skin cancers.
OBJECTIVE: Total regression of large cutaneous lesions less than 1 month with no reoccurrence.
METHODS: Tumor induction on murine model bearing a 500 mm3 subcutaneous lesion. Increasing dose of gold nanoparticles at fixed initial concentration C0 = 0.3 mg/mL, infused into the tumor then exposition of the region of interest to NIR medical laser to assess the therapy. One or two intratumoral administration(s) were compared to surgery and control, that is no treatment, laser alone or nanoparticles alone.
RESULTS: Gold nanoparticles alone or the NIR laser alone did not induce the tumor regression. The combination of laser and nanoparticles called plasmonic nanophotothermal therapy induced apoptosis. Derma and hypoderm do not show any visible gold nanoparticles and demonstrated a good cicatrization process.
CONCLUSIONS: Plasmonic nanophotothermal therapy using two doses of gold nanoparticles was the only protocol that proved its efficacy on large lesions in 14 days, that is 500 mm3 on a murine model bearing human basal cell carcinoma.
OBJECTIVE: Total regression of large cutaneous lesions less than 1 month with no reoccurrence.
METHODS: Tumor induction on murine model bearing a 500 mm3 subcutaneous lesion. Increasing dose of gold nanoparticles at fixed initial concentration C0 = 0.3 mg/mL, infused into the tumor then exposition of the region of interest to NIR medical laser to assess the therapy. One or two intratumoral administration(s) were compared to surgery and control, that is no treatment, laser alone or nanoparticles alone.
RESULTS: Gold nanoparticles alone or the NIR laser alone did not induce the tumor regression. The combination of laser and nanoparticles called plasmonic nanophotothermal therapy induced apoptosis. Derma and hypoderm do not show any visible gold nanoparticles and demonstrated a good cicatrization process.
CONCLUSIONS: Plasmonic nanophotothermal therapy using two doses of gold nanoparticles was the only protocol that proved its efficacy on large lesions in 14 days, that is 500 mm3 on a murine model bearing human basal cell carcinoma.
摘要：
结论：多发性基底细胞癌（BCC）在暴露于阳光的皮肤区域扩散，包括武器,脸和背。一线治疗仍然是手术切除或Mohs手术。尽管它的复杂性很高，Mohs手术在美国和德国都很好，在美学和癌症方面都取得了很好的效果。光动力疗法（PDT）中使用的局部乳膏去除超过2厘米的大病变仍然具有挑战性。如果这些较大的病变在不到1个月的时间内没有得到治疗，它们可以在皮肤深处生长，从而增加复发的风险和疾病的严重程度。尽管本文研究了这个模型，也就是说，非黑色素瘤皮肤癌是一种预后良好的癌症，该疗法旨在应用于更具侵袭性的黑色素瘤皮肤癌。
目的：大的皮肤病变小于1个月，无复发。
方法:在带有500mm3皮下损伤的鼠模型上诱导肿瘤。在固定的初始浓度C0=0.3mg/mL时增加金纳米颗粒的剂量，注入肿瘤，然后将感兴趣的区域暴露于NIR医用激光以评估治疗。将一次或两次肿瘤内给药与手术和对照进行比较，那不是治疗,单独的激光或单独的纳米粒子。
结果:单独的金纳米颗粒或单独的NIR激光不诱导肿瘤消退。激光和纳米颗粒的组合称为等离子体纳米光热疗法诱导细胞凋亡。真皮和下胚层未显示任何可见的金纳米颗粒，并显示出良好的瘢痕化过程。
结论：使用两种剂量的金纳米颗粒的等离子体纳米光热疗法是唯一证明其在14天内对大病灶有效的方案，在患有人类基底细胞癌的小鼠模型上为500mm3。
目的：大的皮肤病变小于1个月，无复发。
方法:在带有500mm3皮下损伤的鼠模型上诱导肿瘤。在固定的初始浓度C0=0.3mg/mL时增加金纳米颗粒的剂量，注入肿瘤，然后将感兴趣的区域暴露于NIR医用激光以评估治疗。将一次或两次肿瘤内给药与手术和对照进行比较，那不是治疗,单独的激光或单独的纳米粒子。
结果:单独的金纳米颗粒或单独的NIR激光不诱导肿瘤消退。激光和纳米颗粒的组合称为等离子体纳米光热疗法诱导细胞凋亡。真皮和下胚层未显示任何可见的金纳米颗粒，并显示出良好的瘢痕化过程。
结论：使用两种剂量的金纳米颗粒的等离子体纳米光热疗法是唯一证明其在14天内对大病灶有效的方案，在患有人类基底细胞癌的小鼠模型上为500mm3。
