PDF(Pubmed)
Abstract：
Astaxanthin biosynthesis in Haematococcus pluvialis is driven by energy. However, the effect of the flagella-mediated energy-consuming movement process on astaxanthin accumulation has not been well studied. In this study, the profiles of astaxanthin and NADPH contents in combination with the photosynthetic parameters with or without flagella enabled by pH shock were characterized. The results demonstrated that there was no significant alteration in cell morphology, with the exception of the loss of flagella observed in the pH shock treatment group. In contrast, the astaxanthin content in the flagella removal groups was 62.9%, 62.8% and 91.1% higher than that of the control at 4, 8 and 12 h, respectively. Simultaneously, the increased Y(II) and decreased Y(NO) suggest that cells lacking the flagellar movement process may allocate more energy towards astaxanthin biosynthesis. This finding was verified by NADPH analysis, which revealed higher levels in flagella removal cells. These results provide preliminary insights into the underlying mechanism of astaxanthin accumulation enabled by energy reassignment in movement-lacking cells.
摘要：
雨生红球藻中虾青素的生物合成是由能量驱动的。然而,鞭毛介导的能量消耗运动过程对虾青素积累的影响尚未得到很好的研究。在这项研究中,表征了虾青素和NADPH含量的概况，以及有或没有pH休克引起的鞭毛的光合参数。结果表明，细胞形态无明显改变，除了在pH休克治疗组中观察到的鞭毛损失。相比之下,鞭毛去除组中的虾青素含量为62.9%，在4、8和12h分别比对照高62.8%和91.1%，分别。同时,Y（II）增加和Y（NO）减少表明缺乏鞭毛运动过程的细胞可能会分配更多的能量用于虾青素的生物合成。NADPH分析证实了这一发现，这表明鞭毛去除细胞中的水平更高。这些结果为缺乏运动的细胞中的能量重新分配提供了对虾青素积累的潜在机制的初步见解。
