Abstract：
UNASSIGNED: Tuberculosis (TB), primarily caused by Mycobacterium tuberculosis, remains a significant global health concern. Targeted Next-Generation Sequencing (tNGS) has emerged as a rapid and comprehensive diagnostic tool for tuberculosis, offering advantages over traditional methods and serving as an effective alternative for drug susceptibility testing and the detection of drug-resistant tuberculosis.
UNASSIGNED: This study aimed to retrospectively analyze the clinical characteristics of pulmonary tuberculosis patients. After explore the application value of targeted next-generation sequencing technology in this patient population, providing valuable insights for clinical diagnosis and treatment.
UNASSIGNED: In this retrospective study, we analyzed data from 65 patients with laboratory-confirmed tuberculosis admitted to Tianjin Baodi Hospital from November 14, 2020, to February 1, 2023. Patients underwent bronchoalveolar lavage fluid (BALF) testing, including acid-fast staining, culture, and tNGS. Biopsies and histopathological examinations were performed on some patients, along with comprehensive radiological assessments for all.
UNASSIGNED: Among the 65 pulmonary tuberculosis patients, targeted next-generation sequencing detected pathogens in bronchoalveolar lavage fluid with a positivity rate of 93.8%, significantly higher than traditional methods such as acid-fast staining, culture, and pathology. Compared to bronchoalveolar lavage fluid smear, targeted next-generation sequencing demonstrated significantly higher diagnostic sensitivity (98.46% vs. 26.15%) and accuracy (98.46% vs. 26.15%).
UNASSIGNED: Targeted next-generation sequencing, with its high sensitivity and specificity compared to traditional methods, provides unique advantages in detecting pathogens among these patients, highlighting its importance in disease management.
UNASSIGNED: This study aimed to retrospectively analyze the clinical characteristics of pulmonary tuberculosis patients. After explore the application value of targeted next-generation sequencing technology in this patient population, providing valuable insights for clinical diagnosis and treatment.
UNASSIGNED: In this retrospective study, we analyzed data from 65 patients with laboratory-confirmed tuberculosis admitted to Tianjin Baodi Hospital from November 14, 2020, to February 1, 2023. Patients underwent bronchoalveolar lavage fluid (BALF) testing, including acid-fast staining, culture, and tNGS. Biopsies and histopathological examinations were performed on some patients, along with comprehensive radiological assessments for all.
UNASSIGNED: Among the 65 pulmonary tuberculosis patients, targeted next-generation sequencing detected pathogens in bronchoalveolar lavage fluid with a positivity rate of 93.8%, significantly higher than traditional methods such as acid-fast staining, culture, and pathology. Compared to bronchoalveolar lavage fluid smear, targeted next-generation sequencing demonstrated significantly higher diagnostic sensitivity (98.46% vs. 26.15%) and accuracy (98.46% vs. 26.15%).
UNASSIGNED: Targeted next-generation sequencing, with its high sensitivity and specificity compared to traditional methods, provides unique advantages in detecting pathogens among these patients, highlighting its importance in disease management.
摘要：
■结核病（TB），主要由结核分枝杆菌引起,仍然是一个重大的全球健康问题。靶向下一代测序（tNGS）已成为结核病的快速和全面的诊断工具，提供优于传统方法的优势，并作为药物敏感性测试和耐药结核病检测的有效替代方法。
■本研究旨在回顾性分析肺结核患者的临床特点。在探讨了靶向下一代测序技术在该患者人群中的应用价值后,为临床诊断和治疗提供有价值的见解。
■在这项回顾性研究中，我们分析了2020年11月14日至2023年2月1日天津宝地医院收治的65例经实验室确诊的结核病患者的数据.患者接受支气管肺泡灌洗液（BALF）检测，包括耐酸染色，文化,和tNGS。对一些患者进行了活检和组织病理学检查，以及对所有人进行全面的放射性评估。
■在65名肺结核患者中，靶向下一代测序检测支气管肺泡灌洗液中的病原体，阳性率为93.8%，显著高于传统方法，如耐酸染色，文化,和病理学。与支气管肺泡灌洗液涂片相比，靶向下一代测序显示出显著更高的诊断灵敏度(98.46%vs.26.15%）和准确性（98.46%与26.15%）。
■靶向下一代测序，与传统方法相比，具有较高的灵敏度和特异性，在这些患者中检测病原体方面提供了独特的优势，强调其在疾病管理中的重要性。
■本研究旨在回顾性分析肺结核患者的临床特点。在探讨了靶向下一代测序技术在该患者人群中的应用价值后,为临床诊断和治疗提供有价值的见解。
■在这项回顾性研究中，我们分析了2020年11月14日至2023年2月1日天津宝地医院收治的65例经实验室确诊的结核病患者的数据.患者接受支气管肺泡灌洗液（BALF）检测，包括耐酸染色，文化,和tNGS。对一些患者进行了活检和组织病理学检查，以及对所有人进行全面的放射性评估。
■在65名肺结核患者中，靶向下一代测序检测支气管肺泡灌洗液中的病原体，阳性率为93.8%，显著高于传统方法，如耐酸染色，文化,和病理学。与支气管肺泡灌洗液涂片相比，靶向下一代测序显示出显著更高的诊断灵敏度(98.46%vs.26.15%）和准确性（98.46%与26.15%）。
■靶向下一代测序，与传统方法相比，具有较高的灵敏度和特异性，在这些患者中检测病原体方面提供了独特的优势，强调其在疾病管理中的重要性。
