PDF(Pubmed)
Abstract：
Midkine is a secreted protein that acts as a growth factor or cytokine involved in cell survival and inflammatory processes. It accumulates in amyloid plaques, which are hallmarks of Alzheimer\'s Disease (AD). The reproducibility of Midkine research would be enhanced if the community had access to well-characterized anti-Midkine antibodies. In this study, we characterized 8 commercial Midkine antibodies for Western blot and immunoprecipitation, using a standardized experimental protocol based on comparing read-outs in a knockout cell line and isogenic parental control. These studies are part of a larger, collaborative initiative seeking to address the antibody reproducibility issue by characterizing commercially available antibodies for human proteins and publishing the results openly as a resource for the scientific community. While use of antibodies and protocols vary between laboratories, we encourage readers to use this report as a guide to select the most appropriate antibodies for their specific needs.
摘要：
中期因子是一种分泌蛋白,其充当参与细胞存活和炎症过程的生长因子或细胞因子。它积聚在淀粉样蛋白斑块中，这是阿尔茨海默病（AD）的标志。如果社区能够获得充分表征的抗中期因子抗体，中期因子研究的可重复性将得到增强。在这项研究中,我们鉴定了8种用于蛋白质印迹和免疫沉淀的商业中期因子抗体，使用基于比较敲除细胞系和等基因亲本对照中的读出的标准化实验方案。这些研究是更大的一部分，通过表征人类蛋白质的市售抗体并公开发布结果作为科学界的资源，寻求解决抗体可重复性问题的协作计划。虽然抗体和方案的使用因实验室而异，我们鼓励读者以本报告为指南，为他们的特定需求选择最合适的抗体。
