PDF(Pubmed)
Abstract：
UNASSIGNED: Since kisspeptin (KISS1) in the hypothalamus is affected by the inhibitory effect of dynorphin, it raises questions about the controlled balance of these 2 neuropeptides in women with and without polycystic ovary syndrome (PCOS).
UNASSIGNED: This study compares the expression levels of KISS1, dynorphin, neurokinin-B, leptin, and neuropeptide-Y in women with and without PCOS.
UNASSIGNED: In this cross-sectional study, the peripheral blood samples of 20 women with PCOS and 20 women without PCOS who referred to Yamin Kencana Clinic, Cipto Mangunkusumo hospital, Jakarta, Indonesia were enrolled from August-December 2022. mRNA relative expression of genes related to the central factors associated with PCOS, such as leptin, neuropeptide-Y, KISS1, tachykinin-3, and prodynorphin (PDYN), in PCOS and non-PCOS populations were examined. Gene quantification was carried out by the quantitative polymerase chain reaction method.
UNASSIGNED: The KISS1/PDYN ratio was significantly higher in the PCOS group than in the control group (p = 0.02), and the PDYN was lower in the PCOS group than the control group (p < 0.001). Moreover, the positive correlation between KISS1 and the KISS1/PDYN ratio was significantly stronger in the PCOS group than in the control group (R = 0.93; p < 0.001 vs. R = 0.66, p < 0.001).
UNASSIGNED: Our results suggest that an increased KISS1/PDYN ratio in PCOS women is related to diminished dynorphin expression. Low expression of the gene encoding dynorphin and a high KISS1/PDYN ratio is highly specific to PCOS.
UNASSIGNED: This study compares the expression levels of KISS1, dynorphin, neurokinin-B, leptin, and neuropeptide-Y in women with and without PCOS.
UNASSIGNED: In this cross-sectional study, the peripheral blood samples of 20 women with PCOS and 20 women without PCOS who referred to Yamin Kencana Clinic, Cipto Mangunkusumo hospital, Jakarta, Indonesia were enrolled from August-December 2022. mRNA relative expression of genes related to the central factors associated with PCOS, such as leptin, neuropeptide-Y, KISS1, tachykinin-3, and prodynorphin (PDYN), in PCOS and non-PCOS populations were examined. Gene quantification was carried out by the quantitative polymerase chain reaction method.
UNASSIGNED: The KISS1/PDYN ratio was significantly higher in the PCOS group than in the control group (p = 0.02), and the PDYN was lower in the PCOS group than the control group (p < 0.001). Moreover, the positive correlation between KISS1 and the KISS1/PDYN ratio was significantly stronger in the PCOS group than in the control group (R = 0.93; p < 0.001 vs. R = 0.66, p < 0.001).
UNASSIGNED: Our results suggest that an increased KISS1/PDYN ratio in PCOS women is related to diminished dynorphin expression. Low expression of the gene encoding dynorphin and a high KISS1/PDYN ratio is highly specific to PCOS.
摘要：
■由于下丘脑中的kisspeptin（KISS1）受到强啡肽的抑制作用的影响，它提出了关于有和没有多囊卵巢综合征（PCOS）的女性中这2种神经肽的受控平衡的问题。
■本研究比较了KISS1、强啡肽、神经激肽B,瘦素,和神经肽-Y在有和没有PCOS的女性中。
■在这项横断面研究中，20名PCOS女性和20名非PCOS女性的外周血样本转诊至YaminKencana诊所，CiptoMangunkusumo医院，雅加达,印度尼西亚从2022年8月至12月注册。与PCOS相关的中枢因子相关基因的mRNA相对表达,比如瘦素,神经肽Y，KISS1，速激肽-3和强啡肽（PDYN），在PCOS和非PCOS人群中进行了检查。通过定量聚合酶链反应方法进行基因定量。
■PCOS组的KISS1/PDYN比值明显高于对照组（p=0.02），PCOS组PDYN低于对照组(p<0.001)。此外，PCOS组KISS1与KISS1/PDYN比值之间的正相关性明显强于对照组（R=0.93；p<0.001vs.R=0.66,p<0.001)。
■我们的结果表明，PCOS女性KISS1/PDYN比值增加与强啡肽表达减少有关。编码强啡肽的基因的低表达和高KISS1/PDYN比率对PCOS具有高度特异性。
■本研究比较了KISS1、强啡肽、神经激肽B,瘦素,和神经肽-Y在有和没有PCOS的女性中。
■在这项横断面研究中，20名PCOS女性和20名非PCOS女性的外周血样本转诊至YaminKencana诊所，CiptoMangunkusumo医院，雅加达,印度尼西亚从2022年8月至12月注册。与PCOS相关的中枢因子相关基因的mRNA相对表达,比如瘦素,神经肽Y，KISS1，速激肽-3和强啡肽（PDYN），在PCOS和非PCOS人群中进行了检查。通过定量聚合酶链反应方法进行基因定量。
■PCOS组的KISS1/PDYN比值明显高于对照组（p=0.02），PCOS组PDYN低于对照组(p<0.001)。此外，PCOS组KISS1与KISS1/PDYN比值之间的正相关性明显强于对照组（R=0.93；p<0.001vs.R=0.66,p<0.001)。
■我们的结果表明，PCOS女性KISS1/PDYN比值增加与强啡肽表达减少有关。编码强啡肽的基因的低表达和高KISS1/PDYN比率对PCOS具有高度特异性。
