PDF(Pubmed)
Abstract：
UNASSIGNED: Pseudomonas aeruginosa is present throughout nature and is a common opportunistic pathogen in the human body. Carbapenem antibiotics are typically utilized as a last resort in the clinical treatment of multidrug-resistant infections caused by P. aeruginosa. The increase in carbapenem-resistant P. aeruginosa poses an immense challenge for the treatment of these infections. Bacteriophages have the potential to be used as antimicrobial agents for treating antibiotic-resistant bacteria.
UNASSIGNED: In this study, a new virulent P. aeruginosa phage, Phage_Pae01, was isolated from hospital sewage and shown to have broad-spectrum antibacterial activity against clinical P. aeruginosa isolates (83.6%). These clinical strains included multidrug-resistant P. aeruginosa and carbapenem-resistant P. aeruginosa. Transmission electron microscopy revealed that the phage possessed an icosahedral head of approximately 80 nm and a long tail about 110 m, indicating that it belongs to the Myoviridae family of the order Caudovirales. Biological characteristic analysis revealed that Phage_Pae01 could maintain stable activity in the temperature range of 4~ 60°C and pH range of 4 ~ 10. According to the in vitro lysis kinetics of the phage, Phage_Pae01 demonstrated strong antibacterial activity. The optimal multiplicity of infection was 0.01. The genome of Phage_Pae01 has a total length of 93,182 bp and contains 176 open reading frames (ORFs). The phage genome does not contain genes related to virulence or antibiotic resistance. In addition, Phage_Pae01 effectively prevented the formation of biofilms and eliminated established biofilms. When Phage_Pae01 was combined with gentamicin, it significantly disrupted established P. aeruginosa biofilms.
UNASSIGNED: We identified a novel P. aeruginosa phage and demonstrated its effective antimicrobial properties against P. aeruginosa in both the floating and biofilm states. These findings offer a promising approach for the treatment of drug-resistant bacterial infections in clinical settings.
UNASSIGNED: In this study, a new virulent P. aeruginosa phage, Phage_Pae01, was isolated from hospital sewage and shown to have broad-spectrum antibacterial activity against clinical P. aeruginosa isolates (83.6%). These clinical strains included multidrug-resistant P. aeruginosa and carbapenem-resistant P. aeruginosa. Transmission electron microscopy revealed that the phage possessed an icosahedral head of approximately 80 nm and a long tail about 110 m, indicating that it belongs to the Myoviridae family of the order Caudovirales. Biological characteristic analysis revealed that Phage_Pae01 could maintain stable activity in the temperature range of 4~ 60°C and pH range of 4 ~ 10. According to the in vitro lysis kinetics of the phage, Phage_Pae01 demonstrated strong antibacterial activity. The optimal multiplicity of infection was 0.01. The genome of Phage_Pae01 has a total length of 93,182 bp and contains 176 open reading frames (ORFs). The phage genome does not contain genes related to virulence or antibiotic resistance. In addition, Phage_Pae01 effectively prevented the formation of biofilms and eliminated established biofilms. When Phage_Pae01 was combined with gentamicin, it significantly disrupted established P. aeruginosa biofilms.
UNASSIGNED: We identified a novel P. aeruginosa phage and demonstrated its effective antimicrobial properties against P. aeruginosa in both the floating and biofilm states. These findings offer a promising approach for the treatment of drug-resistant bacterial infections in clinical settings.
摘要：
■铜绿假单胞菌存在于自然界中，是人体中常见的机会病原体。碳青霉烯类抗生素通常用作临床治疗由铜绿假单胞菌引起的多药耐药感染的最后手段。耐碳青霉烯铜绿假单胞菌的增加对这些感染的治疗提出了巨大的挑战。噬菌体具有用作抗微生物剂用于治疗抗生素抗性细菌的潜力。
■在这项研究中，一种新的毒力铜绿假单胞菌噬菌体,从医院污水中分离出噬菌体_Pae01，并显示对临床铜绿假单胞菌具有广谱抗菌活性（83.6％）。这些临床菌株包括耐多药铜绿假单胞菌和耐碳青霉烯类铜绿假单胞菌。透射电子显微镜显示，噬菌体具有约80nm的二十面体头部和约110m的长尾，表明它属于Caudovirales目的Myoviridae家族。生物学特性分析表明,Phage_Pae01在4~60℃的温度范围和4~10的pH范围内都能保持稳定的活性。根据噬菌体的体外裂解动力学，噬菌体_Pae01表现出强的抗菌活性。最佳感染复数为0.01。Phage_Pae01的基因组总长度为93,182bp，包含176个开放阅读框（ORF）。噬菌体基因组不包含与毒力或抗生素抗性相关的基因。此外,噬菌体_Pae01有效防止生物膜的形成并消除已建立的生物膜。当Phage_Pae01与庆大霉素合用时，它显著破坏了已建立的铜绿假单胞菌生物膜。
■我们鉴定了一种新型的铜绿假单胞菌噬菌体，并证明了其在漂浮和生物膜状态下对铜绿假单胞菌的有效抗微生物特性。这些发现为临床环境中耐药细菌感染的治疗提供了有希望的方法。
■在这项研究中，一种新的毒力铜绿假单胞菌噬菌体,从医院污水中分离出噬菌体_Pae01，并显示对临床铜绿假单胞菌具有广谱抗菌活性（83.6％）。这些临床菌株包括耐多药铜绿假单胞菌和耐碳青霉烯类铜绿假单胞菌。透射电子显微镜显示，噬菌体具有约80nm的二十面体头部和约110m的长尾，表明它属于Caudovirales目的Myoviridae家族。生物学特性分析表明,Phage_Pae01在4~60℃的温度范围和4~10的pH范围内都能保持稳定的活性。根据噬菌体的体外裂解动力学，噬菌体_Pae01表现出强的抗菌活性。最佳感染复数为0.01。Phage_Pae01的基因组总长度为93,182bp，包含176个开放阅读框（ORF）。噬菌体基因组不包含与毒力或抗生素抗性相关的基因。此外,噬菌体_Pae01有效防止生物膜的形成并消除已建立的生物膜。当Phage_Pae01与庆大霉素合用时，它显著破坏了已建立的铜绿假单胞菌生物膜。
■我们鉴定了一种新型的铜绿假单胞菌噬菌体，并证明了其在漂浮和生物膜状态下对铜绿假单胞菌的有效抗微生物特性。这些发现为临床环境中耐药细菌感染的治疗提供了有希望的方法。
