Abstract：
Despite the substantial advancement in developing various hydrogel microparticle (HMP) synthesis methods, emulsification through porous medium to synthesize functional hybrid protein-polymer HMPs has yet to be addressed. Here, the aided porous medium emulsification for hydrogel microparticle synthesis (APME-HMS) system, an innovative approach drawing inspiration from porous medium emulsification is introduced. This method capitalizes on emulsifying immiscible phases within a 3D porous structure for optimal HMP production. Using the APME-HMS system, synthesized responsive bovine serum albumin (BSA) and polyethylene glycol diacrylate (PEGDA) HMPs of various sizes are successfully synthesized. Preserving protein structural integrity and functionality enable the formation of cytochrome c (cyt c) - PEGDA HMPs for hydrogen peroxide (H2O2) detection at various concentrations. The flexibility of the APME-HMS system is demonstrated by its ability to efficiently synthesize HMPs using low volumes (≈50 µL) and concentrations (100 µm) of proteins within minutes while preserving proteins\' structural and functional properties. Additionally, the capability of the APME-HMS method to produce a diverse array of HMP types enriches the palette of HMP fabrication techniques, presenting it as a cost-effective, biocompatible, and scalable alternative for various biomedical applications, such as controlled drug delivery, 3D printing bio-inks, biosensing devices, with potential implications even in culinary applications.
摘要：
尽管在开发各种水凝胶微粒（HMP）合成方法方面取得了重大进展，通过多孔介质进行乳化以合成功能性杂化蛋白-聚合物HMPs尚待解决。这里,水凝胶微粒合成辅助多孔介质乳化(APME-HMS)系统,介绍了一种从多孔介质乳化中汲取灵感的创新方法。该方法利用在3D多孔结构内乳化不混溶相,以实现最佳的HMP生产。使用APME-HMS系统，合成了响应性牛血清白蛋白（BSA）和各种大小的聚乙二醇二丙烯酸酯（PEGDA）HMPs。保持蛋白质结构完整性和功能性使得能够形成用于各种浓度的过氧化氢(H2O2)检测的细胞色素c(cytc)-PEGDAHMPs。APME-HMS系统的灵活性表现在其能够在几分钟内使用低体积（≈50µL）和浓度（100µm）的蛋白质有效合成HMPs，同时保留蛋白质的结构和功能特性。此外,APME-HMS方法生产各种HMP类型的能力丰富了HMP制造技术的调色板，把它作为一个具有成本效益的，生物相容性以及各种生物医学应用的可扩展替代品，如控制药物输送，3D打印生物墨水，生物传感装置，即使在烹饪应用中也有潜在的影响。
