Abstract：
Autism spectrum disorder (ASD) is a neurodevelopmental disorder; the prevalence of which has been on the rise with unknown causes. Alterations in the gut-brain axis have been widely recognized in ASD patients, and probiotics are considered to potentially benefit the rescuing of autism-like behaviors. However, the effectiveness and mechanisms of multiple probiotics on zebrafish models are still not clearly revealed. This study aims to use the germ-free (GF) and conventionally raised (CR) AB wild-type zebrafish and the mutant Tbr1b-/- and Katnal2-/- lines as human-linked ASD animal models to evaluate the effects of multiple probiotics on mitigating developmental and behavioral defects. Results showed that the addition of probiotics increased the basic important developmental indexes, such as body length, weight, and survival rate of treated zebrafish. Moreover, the Lactobacillus plantarum and Lactobacillus rhamnosus affected the behavior of CR zebrafish by increasing their mobility, lowering the GF zebrafish manic, and mitigating transgenic zebrafish abnormal behavior. Moreover, the expression levels of key genes related to gamma-aminobutyric acid (GABA), dopamine (DA), and serotonin (5-HT) as important neuropathways to influence the appearance and development of autism-related disorders, including gad1b, tph1a, htr3a, th, and slc6a3, were significantly activated by some of the probiotics\' treatment at some extent. Taken together, this study indicates the beneficial effects of different probiotics, which may provide a novel understanding of probiotic function in related diseases\' therapy.
摘要：
自闭症谱系障碍（ASD）是一种神经发育障碍；其患病率一直在上升，原因不明。在ASD患者中，肠脑轴的改变已得到广泛认可。和益生菌被认为可能有利于拯救自闭症样行为。然而,多种益生菌对斑马鱼模型的有效性和作用机制尚不清楚。本研究旨在使用无菌（GF）和常规饲养（CR）AB野生型斑马鱼以及突变体Tbr1b-/-和Katnal2-/-系作为人类相关的ASD动物模型，以评估多种益生菌对减轻发育和行为缺陷的影响。结果表明，益生菌的添加增加了基本的重要发育指标，例如身体长度，体重,和处理过的斑马鱼的存活率。此外，植物乳杆菌和鼠李糖乳杆菌通过增加其流动性来影响CR斑马鱼的行为，降低GF斑马鱼的躁狂，减轻转基因斑马鱼的异常行为。此外，γ-氨基丁酸(GABA)相关关键基因的表达水平,多巴胺（DA），和5-羟色胺（5-HT）作为影响自闭症相关疾病的出现和发展的重要神经途径，包括gad1b,tph1a,htr3a,th,和slc6a3，在某种程度上被一些益生菌治疗显著激活。一起来看,这项研究表明了不同益生菌的有益作用，这可能为益生菌在相关疾病治疗中的功能提供了新的理解。
