Abstract:
The development of non-destructive, tomographic imaging systems is a current topic of research in biomedical technologies. One of these technologies is Scanning Laser Optical Tomography (SLOT), which features a highly modular setup with various contrast mechanisms. Extending this technology with new acquisition mechanisms allows us to investigate untreated and non-stained biological samples, leaving their natural biological physiology intact. To enhance the development of SLOT, we aimed to extend the density of information with a significant increase of acquisition channels. This should allow us to investigate samples with unknown emission spectra and even allow for label-fee cell identification. We developed and integrated a hyperspectral module into an existing SLOT system. The adaptations allow for the acquisition of three-dimensional datasets containing a highly increased information density. For validation, artificial test objects were made from fluorescent acrylic and acquired with the new hyperspectral setup. In addition, measurements were made on two different human cell spheroids with an unknown spectra, to test the possibilities of label-free cell identification. The validation measurements of the artificial test target show the expected results. Furthermore, the measurements of the biological cell spheroids show small variations in their tomographic spectrum that allow for label-free cell type differentiation. The results of the biological sample demonstrate the potential of label-free cell identification of the newly developed setup.
摘要:
非破坏性的发展,层析成像系统是生物医学技术研究的当前课题。这些技术之一是扫描激光光学层析成像(SLOT),其特点是一个高度模块化的设置与各种对比机制。通过新的采集机制扩展这项技术,使我们能够研究未经处理和未染色的生物样品,保持其自然的生物生理学完好无损。为了加强SLOT的发展,我们的目标是通过显着增加获取渠道来扩展信息的密度。这应该使我们能够研究具有未知发射光谱的样品,甚至允许进行标签费细胞鉴定。我们开发了一个高光谱模块并将其集成到现有的SLOT系统中。适应允许获取包含高度增加的信息密度的三维数据集。对于验证,人工测试对象由荧光丙烯酸制成,并用新的高光谱装置获得。此外,对两个不同的人类细胞球状体进行了测量,光谱未知,测试无标记细胞识别的可能性。人工测试目标的验证测量显示了预期的结果。此外,生物细胞球状体的测量显示其层析图谱的微小变化,允许无标记细胞类型的分化。生物样品的结果证明了新开发的装置的无标记细胞鉴定的潜力。