Abstract：
BmNPV is a pathogen that infects silkworms exclusively. Although the interaction between BmNPV and the silkworm has been widely noticed and studied, its specific mechanism has still not been elucidated. In this study, we investigated whether BmNPV infection induces the onset of host cell autophagy to enhance viral replication. We observed a significant increase in double- or single-membrane vesicles and an accumulation of enhanced green fluorescent protein eGFP-ATG8 spots in virus-infected cells 72 h after BmNPV infection, accompanied by a conversion of ATG8 to ATG8-PE. In addition, we observed changes in the mitochondrial morphology of BmN cells after BmNPV infection by transmission electron microscopy. By detecting the mitochondrial membrane potential, we found that BmNPV infection resulted in the decrease of mitochondrial membrane potential, and that eGFP-ATG8 was able to co-localise with mitochondria after virus infection of the cells. Moreover, the use of drugs to regulate the occurrence of autophagy affects the replication of cellular BmNPV. Our data demonstrates that BmNPV infection induces host cell autophagy and leads to cellular mitochondrial damage, which in turn may lead to mitochondrial autophagy, and that BmNPV-induced host autophagy promotes its replication in cells. These findings will provide clues for further understanding of host-virus interactions.
摘要：
BmNPV是仅感染家蚕的病原体。尽管BmNPV与家蚕之间的相互作用已被广泛关注和研究,其具体机制尚未阐明。在这项研究中,我们研究了BmNPV感染是否诱导宿主细胞自噬的发生以增强病毒复制.我们观察到在BmNPV感染72小时后，在病毒感染的细胞中，双膜或单膜囊泡的显着增加以及增强的绿色荧光蛋白eGFP-ATG8斑点的积累，伴随着ATG8到ATG8-PE的转换。此外,通过透射电镜观察BmNPV感染后BmN细胞线粒体形态的变化。通过检测线粒体膜电位,我们发现BmNPV感染导致线粒体膜电位降低，并且eGFP-ATG8能够在病毒感染细胞后与线粒体共定位。此外，使用药物调控自噬的发生影响细胞BmNPV的复制。我们的数据表明，BmNPV感染诱导宿主细胞自噬并导致细胞线粒体损伤，这反过来可能导致线粒体自噬，BmNPV诱导的宿主自噬促进其在细胞中的复制。这些发现将为进一步理解宿主-病毒相互作用提供线索。
