Abstract：
Deoxynivalenol-3-glucoside (D3G), the masked form of the important mycotoxin deoxynivalenol (DON), displays potential toxicity but is difficult to control owing to the lack of rapid detection methods. Herein, an innovative molecularly imprinted polymer (MIP)-based electrochemical sensor was developed for the rapid detection of D3G. MIP, an efficient recognition element for D3G, was electropolymerized using o-phenylenediamine based on a surface functional monomer-directing strategy for the first time. CeO2, which contains both Ce3+ and Ce4+ oxidation states, was introduced as a nanozyme to catalyze H2O2 reduction, while Mn doping generated more oxygen vacancies and considerably improved the catalytic activity. Mn-CeO2 also served as a promising substrate material because of its large surface area and excellent conductivity. Under optimal conditions, a good linear relationship was observed for D3G detection over the concentration range of 0.01-50 ng/mL. The proposed sensor could detect D3G down to 0.003 ng/mL with excellent selectivity, even distinguishing its precursor DON in complex samples. The sensor exhibited acceptable stability with high reproducibility and accuracy, and could successfully determine D3G in grain samples. To the best of our knowledge, this is the first electrochemical sensing platform for rapid D3G detection that can easily be expanded to other masked mycotoxins.
摘要：
脱氧雪腐镰刀菌烯醇-3-葡萄糖苷（D3G），重要的霉菌毒素脱氧雪腐镰刀菌烯醇（DON）的掩蔽形式，显示潜在的毒性，但由于缺乏快速检测方法而难以控制。在这里，开发了一种创新的基于分子印迹聚合物（MIP）的电化学传感器，用于快速检测D3G。MIP,D3G的有效识别元素，首次使用基于表面功能单体导向策略的邻苯二胺进行电聚合。同时含有Ce3+和Ce4+氧化态的CeO2,作为纳米酶被引入以催化H2O2还原，Mn掺杂产生更多的氧空位，大大提高了催化活性。Mn-CeO2由于其大的表面积和优异的导电性，也是一种有前途的衬底材料。在最优条件下,在0.01-50ng/mL的浓度范围内，D3G检测呈良好的线性关系。所提出的传感器可以检测D3G低至0.003ng/mL，具有优异的选择性，甚至在复杂样品中区分其前体DON。该传感器具有可接受的稳定性和高重现性和准确性，并能成功测定谷物样品中的D3G。据我们所知,这是第一个用于快速D3G检测的电化学传感平台，可以轻松扩展到其他被掩盖的真菌毒素。
