Abstract：
Passiflora cincinnata is a Passifloraceae typical of the Caatinga, a biome unique to Brazil. It has various pharmacological properties associated with its high flavonoid content. Vitexin, isovitexin, orientin, isoorientin and derivatives are the main chemical and pharmacological markers for this plant. Although flavonoids enriched-extracts have been widely applied in phytocosmetics, especially in sunscreen formulations, the use of P. cincinnata as a photoprotective ingredient remains unexplored. Different hydro-alcoholic extracts were prepared and their antioxidant and photoprotective activities were evaluated by in vitro assays. The most promising extract (Pc-1) was analyzed by HPLC-DAD-ESI-MS/MS. Nine flavonoids were identified as major compounds: isovitexin-7-O-glucoside, isoorientin-2\"O-hexoside, orientin, isoorientin, isovitexin-2\"-O-glucoside, isovitexin-6\"-O-glucoside, isoscoparin and isoquercitrin. Finally, Pc-1 (5 and 10%, v/v) was incorporated into gel formulations, alone or combined to commercial chemical filters (benzophenone-3 and octyl methoxycinnamate). Formulations containing Pc-1 showed high SPFspectrophotometric values. When combined to commercial filters, Pc-1 (5%) potentiated their photoprotective efficacy (p<0.05). A physicochemical characterization indicated no incompatibility or signs of instability after extract incorporation. Altogether, these findings encourage the use of Pc-1 as a photoprotective ingredient or co-adjuvant in sunscreens formulations.
摘要：
西番莲是Caatinga的典型西番莲科，巴西特有的生物群落.它具有与其高类黄酮含量相关的各种药理特性。Vitexin,异维酮,奥伦丁,isoorientin及其衍生物是该植物的主要化学和药理标记。虽然富含黄酮的提取物已广泛应用于植物化妆品中,尤其是在防晒配方中，使用辛辛那塔作为光保护成分仍未探索。制备了不同的水醇提取物，并通过体外测定评估了它们的抗氧化和光保护活性。通过HPLC-DAD-ESI-MS/MS分析了最有前途的提取物（Pc-1）。九种黄酮类化合物被确定为主要化合物：异玻璃化转变素-7-O-葡萄糖苷，isoorientin-2“O-己甙，奥伦丁,isoorientin,异玻璃化蛋白-2“-O-葡萄糖苷，异玻璃化蛋白-6“-O-葡萄糖苷，异黄酮和异槲皮苷。最后,Pc-1(5%和10%，v/v）掺入凝胶制剂中，单独或结合到商业化学过滤器（二苯甲酮-3和辛基甲氧基肉桂酸酯）。含有Pc-1的制剂显示出高的SPF分光光度值。当结合到商业过滤器时，Pc-1(5%)增强了它们的光保护效力(p<0.05)。物理化学表征表明，掺入提取物后没有不相容性或不稳定迹象。总之,这些发现鼓励在防晒制剂中使用Pc-1作为光保护成分或辅助佐剂.
