Abstract：
Tropomyosin (TM) is the main allergen in shrimp (Litopenaeus vannamei). In this study, the effects of allergenicity and structure of TM by glycosylation (GOS-TM), phosphate treatment (SP-TM), and glycosylation combined with phosphate treatment (GOS-SP-TM) were investigated. Compared to GOS-TM and SP-TM, the IgG/IgE binding capacity of GOS-SP-TM was significantly decreased with 63.9 ± 2.0 and 49.7 ± 2.7%, respectively. Meanwhile, the α-helix content reduced, surface hydrophobicity increased, and 10 specific amino acids (K30, K38, S39, K48, K66, K74, K128, K161, S210, and K251) were modified by glycosylation on six IgE linear epitopes of GOS-SP-TM. In the BALB/c mice allergy model, GOS-SP-TM could significantly reduce the levels of specific IgE, IgG1, and CD4+IL-4+, while the levels of IgG2a, CD4+CD25+Foxp3+, and CD4+IFN-γ+ were increased, which equilibrated Th1 and Th2 cells, thus alleviating allergic symptoms. These results indicated that glycosylation combined with phosphate treatment can provide a new insight into developing hypoallergenic shrimp food.
摘要：
原肌球蛋白（TM）是虾（凡纳滨对虾）的主要过敏原。在这项研究中,糖基化对TM变应原性和结构的影响（GOS-TM），磷酸盐处理（SP-TM），和糖基化联合磷酸盐处理（GOS-SP-TM）进行了研究。与GOS-TM和SP-TM相比，GOS-SP-TM的IgG/IgE结合能力明显降低，分别为63.9±2.0和49.7±2.7％，分别。同时,α-螺旋含量减少,表面疏水性增加，在GOS-SP-TM的六个IgE线性表位上通过糖基化修饰了10个特定氨基酸（K30，K38，S39，K48，K66，K74，K128，K161，S210和K251）。在BALB/c小鼠过敏模型中,GOS-SP-TM可显著降低特异性IgE水平,IgG1和CD4+IL-4+，而IgG2a的水平，CD4+CD25+Foxp3+,CD4+IFN-γ+增加，平衡Th1和Th2细胞，从而缓解过敏症状。这些结果表明，糖基化与磷酸盐处理相结合可以为开发低变应原虾食品提供新的见解。
