PDF(Pubmed)
Abstract：
The heart has been the center of numerous transcriptomic studies in the past decade. Even though our knowledge of the key organ in our cardiovascular system has significantly increased over the last years, it is still not fully understood yet. In recent years, extensive efforts were made to understand the genetic and transcriptomic contribution to cardiac function and failure in more detail. The advent of Next Generation Sequencing (NGS) technologies has brought many discoveries but it is unable to comprehend the finely orchestrated interactions between and within the various cell types of the heart. With the emergence of single-cell sequencing more than 10 years ago, researchers gained a valuable new tool to enable the exploration of new subpopulations of cells, cell-cell interactions, and integration of multi-omic approaches at a single-cell resolution. Despite this innovation, it is essential to make an informed choice regarding the appropriate technique for transcriptomic studies, especially when working with myocardial tissue. Here, we provide a primer for researchers interested in transcriptomics using NGS technologies.
摘要：
在过去的十年中，心脏一直是许多转录组学研究的中心。尽管我们对心血管系统的关键器官的了解在过去几年中显著增加，它仍然没有被完全理解。近年来,我们进行了大量努力,以更详细地了解基因和转录组对心脏功能和衰竭的贡献.下一代测序（NGS）技术的出现带来了许多发现，但它无法理解心脏各种细胞类型之间和内部的精细协调相互作用。随着10多年前单细胞测序的出现,研究人员获得了一种有价值的新工具，能够探索新的细胞亚群，细胞-细胞相互作用，以及以单细胞分辨率整合多组学方法。尽管有这种创新,就转录组学研究的适当技术做出明智的选择是至关重要的，尤其是在处理心肌组织时。这里,我们为使用NGS技术对转录组学感兴趣的研究人员提供了引物。
